Cultivation of Detoxification Seedlings and Optimization of the Rapid Propagation System of Southern Medicine Morinda officinalis How

doi:10.3969/j.issn.1000-2561.2023.05.012

Abstract

Abstract:

Morinda officinalis How is a plant of the Rubiaceae, whose dried roots are used as medicine It can strengthen bones and muscles, nourish kidney-yang and remove wind and moisture. It is mainly distributed in Guangdong, Guangxi, Fujian and Hainan provinces. It is one of the four major southern medicines in China. In the main production areas of China, it is mainly propagated by cuttings. In the process of variety breeding, it is often disturbed by plant viruses. The viruses are transmitted and accumulated in plants through asexual propagation of plants, resulting in the inhibition of plant growth and the decline of yield and quality. Therefore, for the purpose of tissue culture detoxification of M. officinalis, the tissue culture detoxification research of M. officinalis was carried out. Thereby improving the yield and quality of M. officinalis, which is beneficial to the protection of M. officinalis species. It is of great practical significance to provide technical support for quality resources and the factory-based seedlings of M. officinalis. Using the stem segments as the explants, an efficient and stable M. officinalis tissue rapid propagation system was established, and suitable plant regeneration methods were screened out. Using 1/2MS as the basic medium, M. officinalis stem axillary buds were used to induce clustered buds to obtain the virus-free seedlings and successfully domesticated and transplanted, the virus detection of M. officinalis tissue culture seedlings and transplanted seedlings were carried out by PCR technology. The semi-lignified stem segments as the explants were suitable to induce axillary buds, the most suitable medium was 1/2MS+6-BA 0.2 mg/L, and the axillary bud induction rate was 70%. Taking the axillary buds to induce fascicled bud, the most suitable medium was 1/2MS+6-BA 0.2 mg/L, and the fascicled bud induction rate was 86.36%. The suitable medium for rooting was 1/2MS+IBA 0.5 mg/L, with a root rate of 100%. The tissue culture seedlings were transplanted in the 1∶1 peat soil-perlite matrix for 8 days with the highest survival rate, reaching 93.3%. The reverse transcript PCR was used to determine the Cucumber mosaic virus isolate M. officinalis How (CMVMO) in the tissue cultured seedlings and transplanted seedlings, the plantlets of M. officinalis How detoxified was obtained. In this study, virus-free seedlings of M. officinalis were successfully cultivated through direct organogenesis, and an in vitro rapid propagation and regeneration system of M. officinalis was established, which would not only improve the yield and quality of M. officinalis, but also provide a reference for virus-free rapid propagation and commercial production and seedling raising of M. officinalis.

Key words: Morinda officinalis How, detoxification, rapid propagation, direct organogenesis, virus detection

CLC Number:

R285

CHEN Zi’en, FENG Chong, LUO Zhenhua, LIU Mengyun, DING Ping. Cultivation of Detoxification Seedlings and Optimization of the Rapid Propagation System of Southern Medicine Morinda officinalis How[J]. Chinese Journal of Tropical Crops, 2023, 44(5): 968-976.

Figures/Tables 10

References 22

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编号 No.	部位 Position	消毒时间 Disinfection time/min	接种数 Number of inoculation	污染率 Pollution rate/%	成活率 Survival rate/%
1	幼嫩茎段	5.5	20	84.68±1.221^abc	10.32±9.014^kl
2		6.0	17	88.89±9.624^ab	5.56±9.624^kl
3		7.0	15	73.33±11.54^bcdef	26.67±11.550^fghij
4		8.0	23	39.28±3.089^hijkl	35.12±9.165^cdefghij
5	半木质茎段	5.5	21	47.62±8.244^defghijkl	47.62±8.244^abcdefgh
6		6.0	23	44.05±11.34^efghijkl	48.21±9.941^abcdefgh
7		7.0	26	42.13±4.007^ghijkl	38.42±5.612^abcdefghij
8		8.0	13	38.33±12.58^hijkl	30.00±8.660^efghij
9	全木质茎段	5.5	26	61.58±5.621^cdefgh	50.00±5.560^abcdefg
10		6.0	22	58.93±3.094^cdefghi	49.94±7.230^abcdefg
11		7.0	19	58.72±13.75^cdefghi	42.06±8.364^abcdefghi
12		8.0	25	56.48±10.43^defghij	36.11±2.408^abcdefghij

编号 No.	部位 Position	消毒时间 Disinfection time/min	接种数 Number of inoculation	污染率 Pollution rate/%	成活率 Survival rate/%
1	幼嫩茎段	5.5	20	84.68±1.221^abc	10.32±9.014^kl
2		6.0	17	88.89±9.624^ab	5.56±9.624^kl
3		7.0	15	73.33±11.54^bcdef	26.67±11.550^fghij
4		8.0	23	39.28±3.089^hijkl	35.12±9.165^cdefghij
5	半木质茎段	5.5	21	47.62±8.244^defghijkl	47.62±8.244^abcdefgh
6		6.0	23	44.05±11.34^efghijkl	48.21±9.941^abcdefgh
7		7.0	26	42.13±4.007^ghijkl	38.42±5.612^abcdefghij
8		8.0	13	38.33±12.58^hijkl	30.00±8.660^efghij
9	全木质茎段	5.5	26	61.58±5.621^cdefgh	50.00±5.560^abcdefg
10		6.0	22	58.93±3.094^cdefghi	49.94±7.230^abcdefg
11		7.0	19	58.72±13.75^cdefghi	42.06±8.364^abcdefghi
12		8.0	25	56.48±10.43^defghij	36.11±2.408^abcdefghij

部位 Position	外植体个数 Number of explants	平均腋芽诱导数 Average number of axillary buds induced	腋芽生长情况 Axillary bud growth
幼嫩茎段	30	1.37±0.56^bc	10~15 d长出白色芽点，每个生长点长一个芽点，顶芽大多只有一个生长点
半木质化茎段	30	1.90±0.55^a	10~15 d长出白色芽点，每个生长点长一个芽点，有时可见多个
全木质化茎段	30	1.17±0.38^bc	15~20 d萌芽，每个生长点长一个腋芽，部分生长点不萌芽

部位 Position	外植体个数 Number of explants	平均腋芽诱导数 Average number of axillary buds induced	腋芽生长情况 Axillary bud growth
幼嫩茎段	30	1.37±0.56^bc	10~15 d长出白色芽点，每个生长点长一个芽点，顶芽大多只有一个生长点
半木质化茎段	30	1.90±0.55^a	10~15 d长出白色芽点，每个生长点长一个芽点，有时可见多个
全木质化茎段	30	1.17±0.38^bc	15~20 d萌芽，每个生长点长一个腋芽，部分生长点不萌芽

培养基 Culture medium	植物生长调节剂 Plant growth regulator	腋芽诱导率 Rate of axillary buds induced/%	腋芽生长情况 Axillary bud growth
MS		56.67±23.09^a	芽苗瘦弱、生长状况不佳
MS	0.2 mg/L 6-BA	73.33±11.54^a	芽苗出现玻璃化现象
1/2 MS	0.2 mg/L 6-BA	70.00±10.00^a	芽苗生长状况良好