Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (5): 955-967.DOI: 10.3969/j.issn.1000-2561.2023.05.011

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Screening and Evaluation of Salt Tolerance in the Bud Stage of Centrosema pubescens Resources

WANG Yanru1, YUAN Bingchen2, SUN Yuting2, WANG Zhiyong1, YU Daogeng3,*()   

  1. 1 College of Forestry, Hainan University, Haikou, Hainan 570228, China
    2 College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    3 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2022-07-12 Revised:2022-08-16 Online:2023-05-25 Published:2023-06-07
  • Contact: YU Daogeng


Centromea pubescens is an important tropical leguminous forage. It is of great significance to study the salt tolerance mechanism of the plant for breeding excellent varieties and improving forage yield. In order to study the difference of salt tolerance of different seeds of C. pubescens in the germination period, the salt tolerance of 75 germplasms was identified in the germination period. NaCl with 150 mmol/L, and 14 indexes such as germination rate, germination potential, root length, bud length and fresh weight were measured, and membership function, principal component analysis, correlation analysis and cluster analysis were carried out to comprehensively evaluate the salt tolerance of germplasm resources of pteromea and to screening salt tolerant germplasm. Under salt stress concentration of 150 mmol/L, the coefficient of variation of relative root shoot ratio was the highest (67.75%), which could be used as one of the evaluation indicators of salt tolerance. The correlation analysis showed that there was a very significant (P<0.01) relationship between any two germination indicators of the germination rate and germination potential, and the other indicators were related to each other to a certain extent. Four principal component as the main component analysis of the direction of the cumulative contribution rate of 83.17% could replace most of the information of all indicators, comprehensively reflecting the germination characteristics during germination under salt stress, and determine the germination index, relative root shoot ratio, relative germination rate and root shoot ratio as the evaluation indicators of salt tolerance through the load matrix. The average membership function and comprehensive score were comprehensively evaluated for salt tolerance, and the 75 germplasm resources were divided into four categories by clustering based on the comprehensive score value of salt tolerance (Z), including 1 highly salt tolerant germplasm (Centrosema 050321057), 17 salt tolerant germplasm, 7 low salt tolerant germplasm and 50 salt sensitive germplasm. In this study, the salt tolerance evaluation system and material evaluation and screening methods were established, which could provide a basis for the study of salt tolerance and salt tolerance gene screening of the genus.

Key words: Centrosema pubescens, correlation analysis, principal component analysis, membership function, cluster analysis

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