Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (5): 894-904.DOI: 10.3969/j.issn.1000-2561.2023.05.003

• Omics & Biotechnology • Previous Articles     Next Articles

Identification of Auxin Receptor Gene TIR1 Family in Cyperus esculentus and the Expression Analysis in Response to Salt Stress and Exogenous IBA

LI Xinru, GAO Yu, MIAO Shunan, LI Teng, DONG Shuyan, SHI Xianfei, XUE Jin’ai, JI Chunli(), LI Runzhi()   

  1. College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China
  • Received:2022-08-02 Revised:2022-10-21 Online:2023-05-25 Published:2023-06-07
  • Contact: *JI Chunli,E-mail:jichunli@sxau.edu.cn;LI Runzhi,E-mail:rli2001@126.com


Cyperus esculentus is a special oil crop, with its underground tuber accumulating a large amount of oil, and also having the advantages of wide adaptability and strong stress resistance. This crop is a superior germplasm material for studying plant stress resistance and oil enrichment in the underground vegetative organs. Transport inhibitor response protein 1 (TIR1) can regulate plant growth and development and abiotic stress response as an auxin receptor. However, few reports on CeTIR1 have been reported. In this study, four TIR1 genes, namely CeTIR1-1, CeTIR1-2, CeTIR1-3 and CeTIR1-4, were identified from C. esculentus transcriptome data and the ORFs were further cloned by RT-PCR. All the four CeTIR1 proteins contained a typical F-box protein domain, AMN1 superdomain and Transp_inhibit domain, revealed by bioinformatics analysis. No significant difference was detected among the four CeTIR1 proteins in regarding sequence length, relative molecular weight, isoelectric point and other physicochemical properties. The four CeTIR1s were closely related to TIR1s derived from plants of Cyperaceae, Oleaceae and Cruciferae in the evolution. Gene expression profiling showed that the expression levels of CeTIR1 family genes were significantly different in different tissues of C. esculentus, with the highest expression in tuber tissues and lower expression in root and leaf tissues. The antioxidant enzyme activity and MDA content of C. esculentus seedlings increased under salt stress. After adding exogenous IBA treatment, the antioxidant enzyme activity of C. esculentus seedlings under salt stress further increased whereas the MDA content decreased. This indicated that IBA could alleviate the adverse effects of salt stress on the growth of C. esculentus seedlings. qRT-PCR results showed that expression levels of the four CeTIR1 genes increased in C. esculentus seedlings treated with salt stress, and exogenous addition of IBA could significantly up-regulate the expression of CeTIR1-2 gene. Comprehensive analysis demonstrates that CeTIR1-2 may be an important gene involved in regulating the response of C. esculentus seedlings to salt stress. Together, the results would provide the scientific basis and important reference for exploring the related function genes involved in auxin regulating the growth and development and stress resistance of C. esculentus, as well as the C. esculentus cultivation in the saline-alkali land.

Key words: Cyperus esculentus L., transport inhibitor response protein 1 (TIR1), indole butyric acid (IBA), salt stress, gene expression

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