Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2022, Vol. 43 ›› Issue (12): 2482-2488.DOI: 10.3969/j.issn.1000-2561.2022.12.010

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Microscopic Observations of Gills, Basidiospores and Mycelium in Agaricus brasiliensis

ZHOU Yu1,2, HUANG Zaixing3, BAO Xinglu4, LI Jing2, LIU Penghu2,*()   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    3. Institute of Applied Microbiology, College of Agriculture, Guangxi University / Institute of Edible Fungi, Nanning, Guangxi 530005, China
    4. Wutun Town Rural Revitalization Development Center, Wuyishan, Fujian 354309, China
  • Received:2022-01-12 Revised:2022-03-27 Online:2022-12-25 Published:2023-01-12
  • Contact: LIU Penghu


Agaricus brasiliensis is a precious edible and medicinal mushroom, which is not only rich in nutrition but also has high medicinal value, and its industrial development has a broad prospect. However, the lack of germplasm resources of A. brasiliensis and insufficient knowledge of the genetic background of A. brasiliensis have hindered the development of its hybrid breeding technology, and the existing germplasm resources can hardly meet the practical needs of production. A. brasiliensis strains J1 was used as research materials, it is proposed to investigate the biological properties related to A. brasiliensis by micro-morphological observation of the reproduction-related structures of A. brasiliensis. The microscopic morphological characteristics of gills, basidiospores and mycelium in different growth and development stages were observed by scanning electron microscope and fluorescence microscope. The gills and trama were in the developmental stage and the hyphae lamellae were not obvious, and the gill sheets were not obvious during the morula state. The gill sheets were obviously observed, arranged parallel to each other, composed of trama and the fruiting layers on both sides, which were closely arranged, uniform in thickness, and a small amount of basidia were borne after the coral stage, but the trama was irregularly arranged. During the forming stage, the gills were clear and regular, and the inner veil was not ruptured, the gill space contains basidiospores and disorganized duct-like cells arranged in raised surface, and the duct-like cells in the gill were closely related. During the forming stage, the gill basidium was relatively full and wrinkled. There were 3 or 4 sporozoites on the gill basidia, and there were small connecting filaments between the basidiospores. The observation of basidiospore nuclei by DAPI staining showed that the most basidiospores were dinuclear spores, and a few were mononuclear and enucleated spores. The observation of mycelial nuclei by DAPI staining showed that the mycelium of A. brasiliensis mycelium was multinucleated and had septa, but there was no lock-like association. This study would clarify the microscopic morphological characteristics of gills, basidia, basidiospores and mycelium in different growth and development stages of A. brasiliensis, enrich the knowledge of biological studies and life history processes of A. brasiliensis, and lay a foundation for the hybrid breeding, basidiospore mating types, genetics transformation system of A. brasiliensis.

Key words: Agaricus brasiliensis, gills, basidiospore, nucleus

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