Identification and Evolutionary Analysis of Maize DUF1685 Gene Family
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2022, Vol. 43 ›› Issue (12): 2431-2442.DOI: 10.3969/j.issn.1000-2561.2022.12.005

• Omics & Biotechnology • Previous Articles     Next Articles

Identification and Evolutionary Analysis of Maize DUF1685 Gene Family

LUO Xuan1, LIN Zhengyu1, CHEN Zhang1, LEI Bo1, LI Jie1, QUAN Jinying1, LIU Hailan2,*()   

  1. 1. Agricultural Information and Rural Economy Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, China
    2. Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
  • Received:2022-04-24 Revised:2022-05-23 Online:2022-12-25 Published:2023-01-12
  • Contact: LIU Hailan

Abstract:

A total of 211 DUF1685 gene family members in 16 monocotyledonous and dicotyledon and Selaginella moellendorffii were identified in this study. Analysis of protein physicochemical properties showed that the amino acid sequences of 211 DUF1685 genes ranged in length from 83 to 1071 aa, molecular weights from 9.2 to 116.3 kDa, and theoretical isoelectric points from 3.66 to 11.90. Phylogenetic evolution analysis showed that the DUF1685 gene family were divided into three subfamilies (Class I, Class II and Class III). The events of A1 and A2, B1 and B2 in the Class I and Class II subfamilies occurred after the differentiation of monocotyledonous plants, and A1 and B2 branches (members were all dicotyledonous genes) had gene expansion phenomenon. The results of conserved motifs and gene structure analysis showed that the conserved motifs among members of the same subfamily were similar. Most of the 211 DUF1685 family genes contained one intron, and a few genes contained two or more introns or no introns. Selection pressure analysis showed that the related loci of DUF1685 family genes were positively selected during the evolution process, which may be the reason for the functional changes of some genes. Collinearity analysis indicated that fragment duplication events may be a potential driver of DUF1685 gene amplification and evolution in the maize. The results of cis-element analysis in the gene promoter region showed that the promoter region of maize DUF1685 gene contained light-responsive elements, ABA and cis-acting elements related to abiotic stress. It was speculated that the genes may be involved in the response of maize to abiotic stress. Transcriptome data analysis revealed that the maize DUF1685 gene was associated with the growth and development of specific tissues (mature pollen). This study explored the function and evolution of the maize DUF1685 gene family in various aspects to provide a theoretical reference for the in-depth study of the biological function and evolutionary relationship of the maize DUF1685 gene in the future.

Key words: DUF1685 gene, gene identification, evolutionary analysis

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