Identification of P-type ATPase Gene Family and Analysis of Expression Pattern in Response to Antagonistic N-1 During Postharvest Storage in Litchi
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2022, Vol. 43 ›› Issue (11): 2366-2374.DOI: 10.3969/j.issn.1000-2561.2022.11.021

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Identification of P-type ATPase Gene Family and Analysis of Expression Pattern in Response to Antagonistic N-1 During Postharvest Storage in Litchi

WANG Xin1, SHAO Yuanzhi2, TANG Yue2, LI Wen1,*()   

  1. 1. Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, China
    2. School of Life Sciences, Hainan University, Haikou, Hainan 570228, China
  • Received:2022-02-25 Revised:2022-04-04 Online:2022-11-25 Published:2022-12-12
  • Contact: LI Wen
  • About author:*LI Wen,E-mail:liwen9-210@163.com

Abstract:

P-type ATPase is an important membrane transporter in plants. It plays a significant role in plant energy metabolism and acid metabolism. In addition, the enzyme is widely involved in plant ion transport, stress resistance, cell signal transduction and other life activities. However, p-type ATPase gene family has not been comprehensively analyzed in litchi or other sapindaceae plants. In this study, based on the transcriptome data of ‘Feizixiao’ litchi, 28 p-type ATPase genes were identified through physiological and biochemical analysis, bioinformatics analysis and expression analysis, to explore the potential function of P-type ATPase during postharvest storage of litchi. The structure and phylogenetic analysis showed that the gene could be divided into 5 subfamilies, the genes in the same subfamily had similar gene structure and motifs. The secondary structure of protein was mainly consisted of α -helix, and the N-terminal domain of LcPAs was more conserved than the C-terminal domain. Subcellular localization prediction analysis showed that LcPAs were mostly located in mitochondria (21/28). According to transcriptome analysis, the up-regulated genes of P type ATPase family genes in litchi were significantly higher than the down-regulated genes during postharst storage, which suggesting that they may play a positive role in the resistance of quality deterioration of litchi after harvest. As a safe and healthy preservation technology, the mechanism of antagonistic biological control is very complex. Results showed that the antagonistic N-1 treatment could effectively inhibit the browning and disease occurrence of litchi fruits after harvest. Combined with RT-qPCR results, it was found that antagonistic N-1 treatment could induce up-regulated expression of LcPA1, LcPA7, LcPA8 and LcPA19 during the whole storage process, which further indicating that P-type ATPase family genes would play an important role in the antagonistic N-1 inhibition of litchi lesions. This study would provide a reference for understanding the evolution and biological function analysis of p-type ATPase gene family in litchi, it also provide new evidence for further understanding the molecular regulation mechanism of antagonistic N-1 preservation mechanism.

Key words: litchi, p-type ATPase gene family, transcriptome, antagonist N-1

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