Ultra-high Pressure Assisted Extraction and Antioxidant Activity of Polysaccharide from Moringa oleifera Seeds

doi:10.3969/j.issn.1000-2561.2022.10.020

Abstract

Abstract:

Ultra-high pressure assisted extraction (UHP) is a new green and high efficient extraction technology for natural products, and the biological activity of the natural products can be kept as much as possible. With Moringa oleifera seeds powder as the experimental material, the water-soluble polysaccharide was extracted by a new ultra-high pressure assisted extraction technology. The antioxidant activities of the water-soluble polysaccharide from M. oleifera seed were evaluated by the total antioxidant capacity and DPPH and hydroxyl radicals scavenging ability. Taking the yield of polysaccharide as an evaluation index, the effects of pulverization degree, pressure, pressure holding time and ratio of material to liquid on the yield of water-soluble polysaccharide from M. oleifera seed were investigated by the single factor experiment method. The orthogonal experiment design method was used to get the optimum ultra-high pressure assisted extraction conditions of polysaccharide from M. oleifera seed. The experiment results showed that the optimum technological conditions of the polysaccharide from M. oleifera seed were determined as follows: holding pressure of 6 min, solid-liquid ratio of 1:15 (g/mL), extraction pressure of 100 MPa and degree of pulverization of 100 mesh. The yield of the polysaccharide from M. oleifera seed under this optimum condition was 0.346%. The experiment results also showed that the scavenging abilities for DPPH and hydroxyl radicals increased with the increase of concentration of the polysaccharide from M. oleifera seed and there was a good linear relationship between concentration of the polysaccharide from M. oleifera seed and the radical scavenging ability. The IC₅₀(the concentration when the radical scavenging rate is 50%) of the polysaccharide from M. oleifera seed for DPPH and hydroxyl radicals was 0.0439 g/L and 0.1666 g/L respectively, and the antioxidant ability was lower than that of Vc at the same concentration, which indicated that the water-soluble polysaccharide from M. oleifera seed had certain antioxidant capacity within a certain concentration range. The total antioxidant capacity of the water-soluble polysaccharide from M. oleifera seed was 0.0482 mmol/L (Expressed as the equivalent concentration of FeSO₄). The water-soluble polysaccharide from M. oleifera seed can be extracted effectively in lower temperature and shorter time by ultra-high pressure assisted extraction technology compared with hot-water extraction method and the water-soluble polysaccharide from M. oleifera seed has good antioxidant activities and can be exploited and used as a natural antioxidant. The results of this study could provide technical support and reference for the exploration of natural antioxidant and the development and high value comprehensive utilization of M. Oleifera.

Key words: Moringa oleifera seeds, polysaccharide, ultrahigh pressure assisted extraction, antioxidant activity

CLC Number:

S202.1

WANG Biaoshi, HUANG Haoyang, TAN Wenhui, LIU Shumin, ZHANG Shiqi, HAN Zhiping, PENG Yuanhuai. Ultra-high Pressure Assisted Extraction and Antioxidant Activity of Polysaccharide from Moringa oleifera Seeds[J]. Chinese Journal of Tropical Crops, 2022, 43(10): 2132-2138.

Figures/Tables 11

Tab. 1 Orthogonal experiment factors and levels

水平 Level	A时间 Time/min	B压力 Pressure/MPa	C料液比 Material-liquid ratio/(g·mL^-1)
1	2	50	1:15
2	4	100	1:20
3	6	150	1:25

Fig. 1 Standard curve of glucose

Fig. 2 Effect of extraction pressure on yield of polysaccharide from M. loeifera seeds

Fig. 3 Effect of pressure holding time on yield of polysaccharide from M. loeifera seeds

Fig. 4 Effect of material-liquid ratio on yield of polysaccharide from M. loeifera seeds

Fig. 5 Effect of crushing degree on yield of polysaccharide from M. loeifera seeds

Tab. 2 Orthogonal test scheme and results

因素 Factor		水平Level		多糖得率 Polycaccharide yield/%
因素 Factor	A时间 Time	B压力 Pressure	C料液比 Material-liquid ratio	多糖得率 Polycaccharide yield/%
1	1	1	1	0.324
2	1	2	2	0.304
3	1	3	3	0.215
4	2	1	3	0.217
5	2	2	1	0.283
6	2	3	2	0.251
7	3	1	2	0.288
8	3	2	3	0.250
9	3	3	1	0.345
K₁	0.281	0.276	0.317
K₂	0.250	0.279	0.281
K₃	0.294	0.270	0.227
R	0.044	0.009	0.090
主次	C>A>B

Fig. 6 Effect of extraction methods on yield of polysaccharide from M. loeifera seeds

Fig. 7 DPPH radical scavenging results of polysaccharide from M. oleifera seeds

Fig. 8 DPPH radical scavenging results of polysaccharide from M. oleifera seeds

Fig. 9 Standard curve of FeSO4 for kit determination

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