Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2022, Vol. 43 ›› Issue (9): 1945-1952.DOI: 10.3969/j.issn.1000-2561.2022.09.023

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Optimization of Extraction and Determination of Carotenoids from Banana Pulp

FAN Yingkang1,2, LI Li2, ZHAN Ni2, KUANG Mengyu2, DENG Guiming2, LYU Peitao1,*(), SHENG Ou2,*()   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350000, China
    2. Institute of Fruit Science, Guangdong Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Resources Utilization of South Subtropical Fruit Trees, Ministry of Agriculture and Rural Affairs / Guangdong Key Laboratory of Tropical and Subtropical Fruit Trees, Guangzhou, Guangdong 510640, China
  • Received:2021-12-20 Revised:2022-03-02 Online:2022-09-25 Published:2022-10-13
  • Contact: LYU Peitao,SHENG Ou


Large-scale evaluation of banana carotenoids germplasm resources, exploration of varieties with high carotenoid resources and improvement of carotenoid contents in main varieties are of great importance to enhance the competitiveness of banana industry. It is necessary to establish an efficient extraction. Meanwhile, it is as well important to grope a determination system for carotenoids in banana fruit pulp. Therefore, this paper primarily aims to establish an optimized protocol for extraction and determination of carotenoids from banana pulp. In this paper, Ultra Performance Liquid Chromatography (UPLC, Agilent1290) was used to determine carotenoids in banana pulp. By performing on a YMC C30 column at the time when column temperature maintaining at 20℃ and the Ultraviolet detection wavelength at 450 nm, we compared the effects of different ratios of solid-liquid (g:mL=1:6, 1:9, 1:12, 1:15), different constant volume solution (MTBE:methanol, V:V=1:0, 1:1, 1:2), different mobile phase (methanol:acetonitrile, V:V=4:0, 3:1, 2:2, 1:3, 0:4,), and different flow rates (0.6 mL/min, 0.8 mL/min, 1 mL/min, 1.2 mL/min) on the identification and content determination of carotene in banana fruit pulp. The results showed that when the extraction solution of hexane/acetone/anhydrous ethanol was 2:1:1 (V:V:V), the solid-liquid ratio of pulp to extraction reagent was 1:9 (g/mL), ultrasonic crushing was performed for 30 minutes, repeated 3 times and the ratio for constant volume solution methanol/MTBE was 1:1 (V:V), the separation effect of carotenoids component in banana fruit pulp was the best. Moreover, the main composition of carotenoids were efficiently separated and identified when we used the binary mobile phase consisting solvent A (methanol:acetonitrile=1:1) and solvent B (MTBE, 100%), under a linear gradient elution procedure, the injection volume of 10 μL and at the flow rate of 1 mL/min. The optimized system was used to detect the carotenoid contents of different kinds of banana fruit pulp. It was found that the main carotenoid compositions in banana fruit pulp were lutein, α-carotene, β-carotene, and so on. ‘French Sombre’ banana variety with high carotenoid contents contained about 9.79 μg/g, while ‘Zhong Jiao 8’ banana variety with low carotenoid contents was only about 0.201 μg/g. This also indicated that the carotenoid contents in different banana varieties was relatively large different. The method on extraction and determination of carotenoids from banana pulp which was established in this paper, is relatively simple and accurate,, providing an optimal protocol for researchers to study the function of carotenoids in bananas.

Key words: banana (Musa spp.), carotenoids, identification and determination of compositions, ultra performance liquid chromatography, system optimization

CLC Number: