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Chinese Journal of Tropical Crops ›› 2022, Vol. 43 ›› Issue (8): 1636-1645.DOI: 10.3969/j.issn.1000-2561.2022.08.013

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Isolation and Identification of Antagonistic Bacteria BACYitc8-7 Against Deciduous Disease Pathogen (Corynespora cassiicola) and Analysis of Antibacterial Components of Volatile Organic Compounds from Rubber Tree

DAI Liming, LI Lanlan, SHI Yuping, LIU Yixian, DENG Yueye, WU Zhonghua, CAI Zhiying()   

  1. Yunnan Institute of Tropical Crops, Jinghong, Yunnan 666100, China
  • Received:2022-01-27 Revised:2022-03-11 Online:2022-08-25 Published:2022-09-09
  • Contact: CAI Zhiying


Corynespora cassiicola is an important pathogen causing Corynespora leaf fall disease of rubber trees. Chemical fungicides are widely used to prevent and control the disease at present, which brings severe ecological environmental issues in rubber planting areas. Isolating and screening bacteria strains with inhibitory activity against C. cassiicola from rhizosphere soil of rubber trees would provide theoretical foundation for researches and development of biological control agents on Corynespora leaf fall disease. Bacteria with high antagonistic activity was obtained from the rhizosphere soil of rubber trees with leaf fall disease by using dilution plate coating and plate-confrontation, its bacteriostatic diameter was (57.68±0.66)mm. It was observed that nine important plant pathogenic fungi could be inhibited by the strain as well. Based on morphological, physiological, biochemical characteristics and 16S rRNA gene sequence, the strain was basically consistent with Pseudomonas aeruginos, and was named BACYitc8-7. The activity of the volatile compounds of BACYitc8-7 against C. cassiicola was evaluated when LB, NA, Gause, Kim B and PDA were used as nutrition sources. The strongest antagonistic effects were found in Kim B and LB mediums, and the inhibition rate was 43.47%-48.69%. 35 substances, including amines, alcohols, alkenes, phenols, esters and pyrazines were obtained from the antimicrobial components of BACYitc8-7 collected and detected by headspace solid phase micro extraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), and the top five contents were amine, borane dimethyl sulfide complex, isoamyl alcohol, 1-triene and 2-methyl-3-methylthiofuran, respectively.

Key words: Corynespora cassiicola, antagonistic bacteria, isolation and identification, volatile substances, antibacterial components

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