Transcriptome Sequencing Analysis of Leaf Vivipary in Water Lily
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (12): 3443-3450.DOI: 10.3969/j.issn.1000-2561.2021.12.009

• Omics & Biotechnology • Previous Articles     Next Articles

Transcriptome Sequencing Analysis of Leaf Vivipary in Water Lily

SU Qun1, TIAN Min2, LI Chunniu1, LI Xianmin1, LU Jiashi1, HUANG Zhanwen1, LI Jiemei1, BU Zhaoyang1,*(), WANG Hongyan1,*()   

  1. 1. Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
    2. Flower Research Institute, Yunnan Academy of Agricultural Sciences / National Engineering Research Center for Ornamental Horticulture, Kunming, Yunnan 650200, China
  • Received:2021-02-20 Revised:2021-05-13 Online:2021-12-25 Published:2022-01-14
  • Contact: BU Zhaoyang,WANG Hongyan

Abstract:

Leaf vivipary in water lily (Nymphaea) is an important supplement to breeding, which plays an important role in the propagation and adaptability of species. Transcriptome sequencing technology was used to screen and explore genome-wide analysis of regulatory genes and metabolic pathways involved in leaf vivipary in water lily. This study would lay a foundation for further understanding the molecular mechanism of leaf viviparous development. Viviparous N. micrantha (X) and non-viviparous N. colorata (L) were selected as the experimental materials. By using RNA-Seq technology, four stages of leaf development of the leaf stalk and stem and stem join were tested and analyzed using a series of bioinformatics analysis. The result showed that 34 909 (48.65%) DEGs were up-regulated and 36 850 (51.35%) DEGs were down-regulated. DEGs analysis showed that up-regulated genes and down-regulated genes both increased with the leaves development of N. micrantha amd N. colorata. GO and KEGG enrichment analysis of L1-vs-X1 and L4-vs-X4 stage showed that DEGs were mainly enriched in plasmalemma, cytomembrane and extracellular domain cytoderm associated with metabolic process, biosynthesis and stimulus response. Pathway metabolism pathways indicated that DEGs were mainly involved in plant hormone signal transduction, phenylpropanoid biosynthesis, amino acids metabolism, flavonoid biosynthesis, glycerolipid metabolism and cell cycle and other processes. Four transcription factors potentially involved in leaf viviparous development of water lily were cloned based on DEGs analysis.

Key words: water lily, leaf vivipary, transcriptome sequencing, DEGs, metabolic pathway

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