Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (11): 3293-3300.DOI: 10.3969/j.issn.1000-2561.2021.11.031

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Determination of Iodopropynyl Butyl Carbamate in Treated Rubber Wood by High Performance Liquid Chromatography

LI Xiaowen1,2, HAN Liping1, LI Tongtong2, ZHANG Jingpeng1, JIANG Mingliang1,*()   

  1. 1. Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China
    2. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / State Engineering and Technology Research Center for Key Tropical Crops, Haikou, Hainan 571101, China
  • Received:2021-03-08 Revised:2021-04-21 Online:2021-11-15 Published:2021-12-24
  • Contact: JIANG Mingliang


Rubber wood is known to be very susceptible to mold and sap stain fungi attacks because of its extraordinary high starch content. 3-iodo-2-propynyl butyl carbamate (IPBC) is suitable for rubber wood preservation as it is highly effective in mold and sap stain fungi, and environmentally friendly. However, few studies on the quantitation methods of IPBC in treated rubber wood have been reported. To determine IPBC in treated rubber wood accurately, a high-performance liquid chromatography (HPLC) method was developed, and the optimization of extracting IPBC from rubber wood was conducted by comparing the effects of different extraction methods, solvents, and particle sizes of wood powder. The HPLC conditions were as follows: C18 reversed-phase column, acetonitrile-water (55︰45, V/V) as mobile phase, flow rate 1.0 mL/min, detection wavelength 204 nm, the retention time of IPBC about 6.45 min. There was a good linear correlation between the mass concentration of IPBC and the peak area with a correlation coefficient of 0.9998 in the range of 2~80 mg/L. The limit of detection (LOD) was 0.2 mg/L, and the average recovery of spiked samples was 99.61%. The comparison of extraction methods showed that ultrasonic extraction was significantly better than the Soxhlet extraction. Methanol or methanol-water (9︰1, V/V) mixed solution was the best solvent to extract IPBC from wood, but ethyl acetate and petroleum ether were not suitable for extracting. Using methanol-water (9︰1, V/V) as the solvent could eliminate the leading peak, which was helpful to improve the accuracy of the analytical method. The difference in wood powder particle size had little effect on the analysis results of IPBC extracted by ultrasonic. The enormous degradation of IPBC due to high temperature greatly affected the analysis results, therefore, high-temperature conditions should be avoided during the analysis process. With the advantages of low detective limit, good reproducibility, and high accuracy, the developed method may have great potential for the analysis of IPBC in preservative woods.

Key words: rubber wood, high-performance liquid chromatography (HPLC), 3-iodo-2-propynyl butyl carbamate (IPBC), extraction method, treated wood

CLC Number: