Determination of Iodopropynyl Butyl Carbamate in Treated Rubber Wood by High Performance Liquid Chromatography

doi:10.3969/j.issn.1000-2561.2021.11.031

Abstract

Abstract:

Rubber wood is known to be very susceptible to mold and sap stain fungi attacks because of its extraordinary high starch content. 3-iodo-2-propynyl butyl carbamate (IPBC) is suitable for rubber wood preservation as it is highly effective in mold and sap stain fungi, and environmentally friendly. However, few studies on the quantitation methods of IPBC in treated rubber wood have been reported. To determine IPBC in treated rubber wood accurately, a high-performance liquid chromatography (HPLC) method was developed, and the optimization of extracting IPBC from rubber wood was conducted by comparing the effects of different extraction methods, solvents, and particle sizes of wood powder. The HPLC conditions were as follows: C18 reversed-phase column, acetonitrile-water (55︰45, V/V) as mobile phase, flow rate 1.0 mL/min, detection wavelength 204 nm, the retention time of IPBC about 6.45 min. There was a good linear correlation between the mass concentration of IPBC and the peak area with a correlation coefficient of 0.9998 in the range of 2~80 mg/L. The limit of detection (LOD) was 0.2 mg/L, and the average recovery of spiked samples was 99.61%. The comparison of extraction methods showed that ultrasonic extraction was significantly better than the Soxhlet extraction. Methanol or methanol-water (9︰1, V/V) mixed solution was the best solvent to extract IPBC from wood, but ethyl acetate and petroleum ether were not suitable for extracting. Using methanol-water (9︰1, V/V) as the solvent could eliminate the leading peak, which was helpful to improve the accuracy of the analytical method. The difference in wood powder particle size had little effect on the analysis results of IPBC extracted by ultrasonic. The enormous degradation of IPBC due to high temperature greatly affected the analysis results, therefore, high-temperature conditions should be avoided during the analysis process. With the advantages of low detective limit, good reproducibility, and high accuracy, the developed method may have great potential for the analysis of IPBC in preservative woods.

Key words: rubber wood, high-performance liquid chromatography (HPLC), 3-iodo-2-propynyl butyl carbamate (IPBC), extraction method, treated wood

CLC Number:

S782.33

LI Xiaowen, HAN Liping, LI Tongtong, ZHANG Jingpeng, JIANG Mingliang. Determination of Iodopropynyl Butyl Carbamate in Treated Rubber Wood by High Performance Liquid Chromatography[J]. Chinese Journal of Tropical Crops, 2021, 42(11): 3293-3300.

Figures/Tables 11

References 25

[1]	何长辉, 莫业勇, 刘锐金. 中国天然橡胶生产能力预测分析(2019—2025年)[J]. 林业经济问题, 2020, 40(3):320-327.
[2]	蒋明亮, 张厚培. 橡胶木防腐防蛀技术研究概况[J]. 木材工业, 1996(6):21-23, 30.
[3]	Rebecca S L. Final report on the safety assessment of iodopropynyl butylcarbamate (IPBC)[J]. International Journal of Toxicology, 1998, 17(5):1-37.
[4]	Lars J, Judy B, Pierre Y C, et al. Fate, behavior, and aquatic toxicity of the fungicide IPBC in the Canadian environment[J]. Environmental Toxicology, 2000, 15(3):201-213. DOI URL
[5]	Wozniak E, Cubizolles L, Delourme F N, et al. Xylophene AS. Xylophene AS, the challenge of developing a modern antisapstain formulation for the French market[C]// Proceedings of the 31st IRG Annual Meeting. Kona, Hawaii, USA: International Research Group on Wood Protection, 2000: IRG/WP 00-30250.
[6]	Hansen J. IPBC - A new fungicide for wood protection[C]// Proceedings of the 15th IRG Annual Meeting, Stockholm, Sweden: International Research Group on Wood Protection, 1984: IRG/WP 3295.
[7]	Cserjesi A J, Byrne A, Johnson E L. Long-term protection of stored lumber against mould, stain, specifically decay: A comparative field test of fungicidal formulations[C]// Proceedings of the 15th IRG Annual Meeting, Stockholm, Sweden: International Research Group on Wood Protection, 1984: IRG/WP 3281.
[8]	翟炜, 何深远, 崔爱玲, 等. 碘代丙炔基氨基甲酸酯的防腐性能评价[J]. 木材工业, 2014, 28(2):22-25.
[9]	Daniel L C, Wallace E E. Fungicides to prevent sapstain and mold on hardwood lumber[J]. Forest Products Journal, 1981, 31(9):39-42.
[10]	Daniel L C, Wallace E E. Field trials of chemicals to control sapstain and mold on yellow-poplar and southern yellow pine lumber[J]. Forest Products Journal, 1983, 33(10):52-56.
[11]	魏万姝, 覃道春. 4种防霉剂对重组竹性能的影响[J]. 东北林业大学学报, 2011, 39(4):93-95.
[12]	靳肖贝. 纳米埃洛石负载IPBC竹材防霉剂的制备与性能研究[D]. 北京: 中国林业科学研究院, 2018.
[13]	李瑜瑶, 张融, 靳肖贝, 等. 埃洛石纳米管改性竹材防霉涂料的性能研究[J]. 南京林业大学学报(自然科学版), 2016, 40(3):127-132.
[14]	李晓文, 李家宁, 李民等. 碘代丙炔基丁基氨基甲酸酯复配制剂用于橡胶木防霉防蓝变效果检测[J]. 木材工业, 2015, 29(2):42-45.
[15]	Hong L T. Performance of proprietary formulations of anti- sapstain preservatives on Hevea brasiliensis timbers in laboratory tests[C]// Proceedings of the 20th IRG Annual Meeting, Lappeenranta, Finland: International Research Group on Wood Protection, 1989: IRG/WP 3532.
[16]	吴玉銮, 王莉, 罗海英, 等. 超声萃取结合超高效液相色谱法测定皮革中10种防霉剂[J]. 分析测试学报, 2016, 35(9):1116-1120.
[17]	马强, 王超, 白桦, 等. 化妆品中碘丙炔醇丁基氨甲酸酯的高效液相色谱检测及质谱确证[J]. 分析试验室, 2009, 28(10):56-58.
[18]	赵玥, 李琼, 张婉萍, 等. 高效液相色谱法对化妆品中低浓度碘丙炔醇丁基氨甲酸酯(IPBC)的检测[J]. 化学世界, 2011, 52(2):87-91.
[19]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 有机型木材防腐剂分析方法——三唑及苯并咪唑类: GB/T 33021—2016[S]. 北京: 中国标准出版社, 2016.
[20]	肖忠平, 陆继圣. IPBC防腐处理材的防腐性和抗流失性研究[J]. 西北林学院学报, 2010, 25(5):187-190.
[21]	Klara K, Brianna G, Fisher S, et al. Optimization of electrospray ionization for liquid chromatography time-of-flight mass spectrometry analysis of preservatives in wood leachate matrix[J]. Chromatographia, 2019, 82(11):1677-1685. DOI URL
[22]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 造纸原料水分的测定: GB/T 2677.2— 2011[S]. 北京: 中国标准出版社, 2011.
[23]	American Wood Protection Association. Standard method for analysis of propiconazole, tebuconazole and imidacloprid in treated wood products by high performance liquid chromatography with multi-level internal standards: AWPA A48-09[S]. Birmingham, America: AWPA Inc., 2009.
[24]	American Wood Protection Association. Standard method for determination of propiconazole and tebuconazole in wood, in waterborne formulations, and in treating solutions by high performance liquid chromatography: AWPA A28-05[S]. Birmingham, America: AWPA Inc., 2005.
[25]	马红霞, 张燕君, 陈利芳. 固相萃取预处理对木材中戊唑醇含量检测效果的研究[J]. 广东林业科技, 2011, 27(5):31-34.

本底值 Background /(mg·L^-1)	加标量 Spiked level /(mg·L^-1)	测定值 Measured value /(mg·L^-1)	加标回收率 Recovery of spiked samples/%
24.81	40	64.67	99.6
35.74	40	75.51	99.4
22.23	40	62.34	100.3
44.78	40	84.75	99.9
24.98	40	65.09	100.3
27.97	40	68.17	100.5
30.46	20	50.02	97.8
34.24	20	54.06	99.1
41.79	20	61.69	99.5
23.70	20	43.45	98.7
24.50	20	44.42	99.6
26.99	20	47.12	100.6

本底值 Background /(mg·L^-1)	加标量 Spiked level /(mg·L^-1)	测定值 Measured value /(mg·L^-1)	加标回收率 Recovery of spiked samples/%
24.81	40	64.67	99.6
35.74	40	75.51	99.4
22.23	40	62.34	100.3
44.78	40	84.75	99.9
24.98	40	65.09	100.3
27.97	40	68.17	100.5
30.46	20	50.02	97.8
34.24	20	54.06	99.1
41.79	20	61.69	99.5
23.70	20	43.45	98.7
24.50	20	44.42	99.6
26.99	20	47.12	100.6

IPBC载药量 Calculated retention of IPBC/(mg·g^-1)	测定载药量 Measured retention of IPBC/(mg·g^-1)
IPBC载药量 Calculated retention of IPBC/(mg·g^-1)	甲醇 Methanol	乙酸乙酯 Ethyl acetate	石油醚 Petroleum ether
2.693	0.853±0.207	0.658±0.127	0.459±0.094
1.426	0.566±0.089	0.378±0.109	-

IPBC载药量 Calculated retention of IPBC/(mg·g^-1)	测定载药量 Measured retention of IPBC/(mg·g^-1)
IPBC载药量 Calculated retention of IPBC/(mg·g^-1)	甲醇 Methanol	乙酸乙酯 Ethyl acetate	石油醚 Petroleum ether
2.693	0.853±0.207	0.658±0.127	0.459±0.094
1.426	0.566±0.089	0.378±0.109	-

IPBC载药量 Calculated retention of IPBC/(mg·g^-1)	测定载药量 Measured retention of IPBC/(mg·g^-1)
2.931	1.936±0.166
1.492	1.122±0.047