Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 3033-3041.DOI: 10.3969/j.issn.1000-2561.2021.10.038

• Agricultural Ecology & Environmental Protection • Previous Articles     Next Articles

Effect of Streptomyces and Chitosan on the Soil Microbial Activities and Microbiome in Dioscorea oppositifolia L.

LU Jianming, HUANG Xiaolong, WU Wenqiang, XU Yun, XIA Wei, ZHANG Rongping*()   

  1. Hainan University, Haikou, Hainan 570228, China
  • Received:2020-12-15 Revised:2021-02-15 Online:2021-10-25 Published:2021-11-25
  • Contact: ZHANG Rongping

Abstract:

In order to explore the effect of Streptomyces 30702 and chitosan on the soil microbial activity and microbiome in Dioscorea oppositifolia L., the soil enzyme activity development was measured by colorimetric and titration methods, the change of the bacteria and fungi in soil microbiome was detected using the high-throughput sequencing technology and softwares such as Trimmomatic. The results showed that Streptomyces, chitosan, soil original microorganisms and culture time all affected the activity of urease and catalase in the soil significantly. The soil enzyme activity increased as chitosan concentration rose from 0 to 10.0 g/kg. As growing time went on, the soil enzyme activity increased first and then decreased obviously. Chitosan and Streptomyces interacted obviously. Streptomyces and chitosan increased the species abundance of soil bacteria and reduced the diversity of bacteria, increased the relative abundance of beneficial bacteria such as Ideonella and Cellvibrio, but had little effect on the species abundance and diversity of fungi and there was no significant difference between them and CK, the dominant bacteria genera in the soil were Humicola, Cladosporium, Sphingomonas and uncultured_bacterium_c_subgroup_6. Among them, the species abundance of bacteria in the Streptomyces 30702 2.5 g/kg treatment combined with chitosan was the largest, and the diversity of bacteria in the treatment was the least.

Key words: Streptomyces sp., chitosan, Dioscorea oppositifolia L., soil microbial activity, soil microbiome, interaction effect

CLC Number: