Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 3008-3016.DOI: 10.3969/j.issn.1000-2561.2021.10.035

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Comparative Analysis of the Effects of Different Precipitation Agents on Extraction of Plant Protein by Phenol Extract

HUO Yuxin1, GAN Panlu1, WANG Yujing1, ZHANG Xueyan1, WANG Xuchu1,2,*(), XIE Quanliang1,2,*()   

  1. 1. Hainan Normal University / Key Laboratory of Tropical Island Ecology, Ministry of Education, College of Life Sciences, Haikou, Hainan 571158, China
    2. College of Life Science and Agricultural, Shihezi University, Shihezi, Xinjiang 832003, China
  • Received:2020-12-15 Revised:2021-03-08 Online:2021-10-25 Published:2021-11-25
  • Contact: WANG Xuchu,XIE Quanliang


The borax/PVPP/phenol (BPP) protocol is a widespread protein extraction method in plant proteome research. However, BPP extraction of plant protein, combined with different precipitation agents, the precipitation time, yield and purity of extracted protein is strikingly different. In this study, Sesuvium portulacastrum and Para-rubber tree leaves, and rubber latex were used as the research materials. The plant protein was extracted and analyzed with precipitating agents of supersaturated potassium ammonium sulfate alcohol solution, ammonium acetate methanol solution and acetone solution. Using the methods of SDS-PAGE, gel electrophoresis image quality, mass spectrum identification, settling duration and comparative analysis of protein yield, it was showed that the protein bands extracted by the ammonium acetate precipitation method were not evident. Hevea leaves and latex showed a higher yield of protein in acetone solution. S. portulacastrum leaves had a higher yield of protein in supersaturated potassium ammonium sulfate alcohol solution. The clear background of gel electrophoresis figures was found for the supersaturated potassium ammonium sulfate ethanol solution and acetone solution. The comprehensive protein sequence information identified by mass spectrometry had a higher quality of precipitated protein. The research results can optimize the method of extracting high-quality plant protein, and it will be expected to provide references for recalcitrant plant taxa proteomics research in the future.

Key words: protein extraction, Hevea brasiliensis, Sesuvium portulacastrum, precipitant, polypropylene gel electrophoresis, mass spectrometry

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