Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2972-2978.DOI: 10.3969/j.issn.1000-2561.2021.10.030

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Myzus persicae Feeding Effects Salicylic Acid and Jasmonic Acid Signaling Pathways in Aphid-resistant and Aphid-susceptible Pepper Cultivars

FAN Dongzhe1,2,3, CHEN Qing2,3,*(), LIANG Xiao2,3, WU Chunling2,3, LIU Ying2,3, DOU Hongshuang2,3, WU Yan2,3   

  1. 1. School of Plant Protection, Hainan University, Haikou, Hainan 570228, China
    2. Environment and Plant Protection Institute, China Academy of Tropical Agricultural Sciences / Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture and rural affairs / Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, Hainan 571101, China
    3. Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan 572000, China
  • Received:2020-12-28 Revised:2021-02-12 Online:2021-10-25 Published:2021-11-25
  • Contact: CHEN Qing

Abstract:

In order to clarify the important role of salicylic acid and jasmonic acid signal pathways in pepper aphid resistance, the study was based on obtaining genetically stable resistant and susceptible pepper varieties, and systematically carried out the difference analysis of salicylic acid and jasmonic acid content and related gene expression in leaf tissue of the aphid-resistance and aphid-susceptible reference peppers cultivars before and after damaged by Myzus persicae. After damaged by M. persicae, the content of salicylic acid in the aphid-resistant pepper variety ZDC and the aphid-susceptible pepper variety DYJJ increased significantly, while the content of jasmonic acid increased significantly in ZDC. In DYJJ, it decreased significantly and then returned to the pre-damage level. The expression levels of salicylic acid signaling pathway related genes PR1, EDS5, PAD4 could be significantly induced after ZDC and DYJJ damaged by M. persicae, and the expression level of PR1 and EDS5 genes in ZDC was significantly higher in ZDC and DYJJ in the early stage of damage. After ZDC was damaged by green peach aphid, the expression level of the jasmonic acid signaling pathway genes LOX2 and AOC increased significantly, and then gradually decreased to the pre-damage level. The expression of the genes in DYJJ was significantly inhibited and also significantly lower than that in ZDC. The results of this study indicate that pepper-resistant varieties can simultaneously activate salicylic acid and jasmonic acid signal pathways to resist the damage of green peach aphid, while the aphid-susceptible pepper variety activates the salicylic acid pathway and significantly inhibits the jasmonic acid pathway, which is beneficial to the green peach aphid infestation. This study initially revealed the molecular mechanism of pepper’s aphid resistance from the above two defense signal pathways.

Key words: Myzus persicae, aphid-resistant and aphid-susceptible reference standard pepper cultivar, salicylic acid, jasmonic acid

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