Effect of Exogenous Gibberellin Signal on Sugarcane Tillering and Its Endogenous Hormones

doi:10.3969/j.issn.1000-2561.2021.10.026

Abstract

Abstract:

Previous studies found that the exogenous GA signal affected sugarcane tillering process. In order to understand the effect of GA signal on the content of endogenous hormones in leaves at different time of tillering stage of sugarcane, this experiment used the main sugarcane variety ‘Guitang 42’ with excellent comprehensive traits as the plant material, and different exogenous GA signal regulators were used to soak the seedcanes with water treatment as the control (CK). The endogenous hormones indole-3-acetic acid (IAA) and gibberellins (GA), ethylene (ETH), cytokinin (CTK), abscisic acid (ABA) and brassinosteroids (BR) content in +1 leaves (main seedling + tiller seedling) at the early, full and late tillering stages were analyzed and the correlations between them were statistically tested. The results showed that after soaking sugarcane seedcanes with exogenous GA₃ (Gibberellic acid 3; GA biosynthesis promoter), the total contents of IAA, GA and CTK in the tiller leaves was increased at the tillering stage, and the ETH content at the early stage and the ABA content at late tillering stage were increased. Paclobutracol (Paclobutracol, PP₃₃₃; GA biosynthesis inhibitor) treatment increased the endogenous GA and ABA contents before tillering, and the ETH content at the later tiller leaves, but reduced the CTK and BR contents. Further correlation analysis found that in the treatment with exogenous gibberellin signal enhancer, the endogenous hormones of sugarcane leaves IAA and GA, CTK and BR, CTK and GA showed a significant positive correlation while ABA showed a significant negative correlation with GA but a significant positive correlation with ETH. The exogenous GA signal affected the endogenous hormone system in the leaves of sugarcane at the tillering stage, and the growth-promoting hormones and the growth-inhibiting hormones reached a dynamic equilibrium state, which ultimately led to the difference in the tillering. It is concluded that sugarcane tillering is closely related to the contents of plant endogenous hormones. Exogenous hormones could be used to influence the contents of endogenous hormones, regulate the dynamic balance between hormones, and achieve the purpose of improving sugarcane tiller formation and its stem formation.

Key words: Saccharum officinarum, plant growth regulator, gibberellin, paclobutrazol, seedcane soaking, tiller, endogenous hormones

CLC Number:

S533

LI Zhengying, QIU Lihang, YAN Haifeng, ZHOU Huiwen, CHEN Rongfa, FAN Yegeng, LIANG He, WU Jianming. Effect of Exogenous Gibberellin Signal on Sugarcane Tillering and Its Endogenous Hormones[J]. Chinese Journal of Tropical Crops, 2021, 42(10): 2942-2951.

Figures/Tables 7

Fig. 1 Effects of different GA signal treatments on IAA content of sugarcane tillers Different lowercase letters indicate significant difference (P<0.05).

Fig. 2 Effects of different GA signal treatments on GA content of sugarcane tillers Different lowercase letters indicate significant difference (P<0.05).

Fig. 3 Effects of different GA signal treatments on ETH content of sugarcane tillers Different lowercase letters indicate significant difference (P<0.05).

Fig. 4 Effects of different GA signal treatments on CTK content of sugarcane tillers Different lowercase letters indicate significant difference (P<0.05).

Fig. 5 Effects of different GA signal treatments on ABA content of sugarcane tillers Different lowercase letters indicate significant difference (P<0.05).

Fig. 6 Effects of different GA signal treatments on BR content of sugarcane tillers Different lowercase letters indicate significant difference (P<0.05).

Tab. 1 Correlation coefficients between the number of tillers and endogenous hormones in sugarcane

指标Index	IAA	GA	ETH	CTK	ABA	BR
平均分蘖率	-0.258	-0.071	-0.272	-0.209	0.034	-0.284
IAA	1	0.81^**	-0.415	0.995^**	-0.51	0.687^*
GA		1	-0.502	0.824^**	-0.717^*	0.617
ETH			1	-0.433	0.702^*	-0.514
CTK				1	-0.553	0.631
ABA					1	-0.433
BR						1

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