Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2935-2941.DOI: 10.3969/j.issn.1000-2561.2021.10.025

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Determination of Chlorophyll Content and Observation of Ultrastructure in Leaves of Mutants of Artocarpus heterophyllus

ZHOU Dan1,2,3,4, LUO Can1,4, YU Xudong1,2,3,4,*(), CAI Zeping1, WU Fanhua1   

  1. 1. College of Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Hainan University), Ministry of Education, Haikou, Hainan 570228, China
    3. Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan, Haikou, Hainan 570228, China
    4. Hainan Sub-platform of National Forest Genetic Resources Platform, Danzhou, Hainan 571737, China
  • Received:2021-01-15 Revised:2021-03-21 Online:2021-10-25 Published:2021-11-25
  • Contact: YU Xudong

Abstract:

Artocarpus heterophyllus is a tropical fruit, and so far there are few reports on its mutants. In this study, the leaves of A. heterophyllus were used as the experimental materials to explore the possible reasons for the albinism and regreening of the leaves. The contents of chlorophyll and chlorophyll precursors in leaf mutants and normal green leaves were determined by conventional methods. The activity of chlorophyllase was determined by Elisa kit. The ultrastructure of chloroplast was observed by a transmission electron microscope. Chlorophyll a content, chlorophyll b content, total chlorophyll content and carotenoid content produced by different leaf colors were significantly different. There was no significant difference in the content of chlorophyll synthesis precursors between the mutant and the normal green leaves. In the determination of chlorophyll enzyme activity of the leaves, although there was a significant difference in its activity, it had no significant effect on the difference in chlorophyll content. When observing the ultrastructure of chloroplasts in the leaves, it was found that the chloroplasts in the normal green leaves were intact and had a large number, and the internal structure of chloroplasts in the albino leaves and the regreening leaves was defective, because the chloroplast grana construction stage was blocked. Based on the determination of chlorophyll, chlorophyll precursor content and chlorophyll enzyme activity in the leaves, the ultrastructure of chloroplast in the leaves was observed, and the data were compared and analyzed. In this study, it was speculated that during the chlorophyll synthesis stage, the blocked synthesis of chlorophyll by pchlide and the poor chloroplast development led to the albinism and regreening of A. heterophyllus, which would provide a theoretical basis for further comprehensive study of A. heterophyllus mutants.

Key words: Artocarpus heterophyllus, chlorophyll, chlorophyll precursor substance, chlorophyll enzyme activity, ultrastructure

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