Effects of Low Phosphorus Stress on the Growth and Antioxidant System of Stylosanthes

doi:10.3969/j.issn.1000-2561.2021.10.017

Abstract

Abstract:

Using genotype TF291 as the test material, the effects of low phosphorus treatment (5 μmol/L) on the growth, antioxidant and antioxidant enzymes of Stylosanthes were analyzed to explore the response mechanism of the antioxidant system of Stylosanthes under low phosphorus stress. Compared with the control p treatment (250 μmol/L), the low p treatment inhibited the growth of stylox, and the chlorophyll concentration, maximum photochemical efficiency, abovementium and root biomass were significantly reduced (P<0.05). With the increase of the treatment time of low phosphorus, the activity of CAT, POD, SOD, ASP, PAL and the content of flavonoids in leaves were significantly increased at 15 d (P<0.05). The activity of CAT, SOD, ASP, PPO and content of MDA and H₂O₂ in leaves were significantly increased at 30 d (P<0.05). The study would provide an important basis for further exploring the molecular response mechanism of the antioxidant system of Stylosanthes cylindrica under low phosphorus stress.

Key words: Stylosanthes, reactive oxygen species, low phosphorus stress, antioxidant system

CLC Number:

S541

WANG Jinpeng, LIAO Li, LIU Guodao, LUO Lijuan, LIU Pandao, WAMG Zhiyong. Effects of Low Phosphorus Stress on the Growth and Antioxidant System of Stylosanthes[J]. Chinese Journal of Tropical Crops, 2021, 42(10): 2875-2880.

Figures/Tables 5

Fig. 1 Growth performance of Stylosanthes at low phosphorus concentration treatments Different lowercase letters indicate significant difference among different treatments (P<0.05).

Tab.1 Effects of different phosphorus concentrations on total phosphorus content of Stylosanthes

磷处理 Phosphorus treatment	处理时间 Duration of treatment/d	地上部全磷含量 Total P content in shoot/(mg·g^-1)	根部全磷含量 Total P content in root/(mg·g^-1)
LP	15	0.73±0.03^c	0.08±0.005^d
	30	0.79±0.04^c	0.39±0.010^c
HP	15	6.24±0.24^b	0.79±0.030^b
	30	8.17±0.31^a	1.38±0.070^a

Tab. 2 Effects of different phosphorus concentrations on antioxidant content of Stylosanthes

磷处理 Phosphorus treatment	处理时间 Duration of treatment/d	丙二醛含量 Malondialdehyde content/(nmol·g^-1)	过氧化氢含量 Hydrogen peroxide content/(mmol·g^-1)	总酚含量 Total phenol content/(mg·g^-1)	类黄酮含量 Flavonoid content/(mg·g^-1)
LP	15	162.41±11.24^c	103.74±5.49^b	1.08±0.025^b	3.55±0.044^b
LP	30	185.47±12.31^b	161.33±6.62^a	1.06±0.067^b	5.15±0.034^a
HP	15	221.76±14.38^a	110.40±5.18^b	3.57±0.069^a	3.32±0.076^c
HP	30	167.07±10.23^c	109.07±4.91^b	0.94±0.014^c	3.13±0.098^c

Fig. 2 Effects of different phosphorus concentrations on total antioxidant enzyme activity of Stylosanthes Different lowercase letters indicate significant difference among different treatments (P<0.05).

Fig. 3 Effects of different phosphorus concentrations on phenylalanine ammonia-lyase and polyphenol oxidase activities of shoot and root of Stylosanthes Different lowercase letters indicate significant difference among different treatments (P<0.05).

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