Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2859-2868.DOI: 10.3969/j.issn.1000-2561.2021.10.015

• Omics & Biotechnology • Previous Articles     Next Articles

Genome-wide Identification and Expression Analysis of the CAMTA Family in Rubber Tree (Hevea brasiliensis)

LIN Xianzu1,2, XIAO Xiaohu2, YANG Jianghua2, QIN Yunxia2, LONG Xiangyu2, FANG Yongjun2,*()   

  1. 1. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2020-12-28 Revised:2021-03-19 Online:2021-10-25 Published:2021-11-25
  • Contact: FANG Yongjun

Abstract:

Calmodulin-binding transcription activators (CAMTAs) are important proteins with a conserved calmodulin- binding domain, which are widely involved in biological processes such as stress response, growth and development, and hormone signalling in plant. In this study, we identified eight HbCAMTAs screening the genome-wide of the rubber tree and analyzed physicochemical properties, gene structures, regulatory elements, gene expression patterns, and so on. The results showed that all HbCAMTAs were located in the nucleus, and predicted as hydrophilic proteins with molecular weight from 104.34 to 121.99 kD and isoelectric points between 5.32 and 7.83. The sequence homology analysis showed that HbCAMTAs could be divided into four subclades, and each one had similar gene structure, conserved domains. Furthermore, each HbCAMTA was potentially regulated by at least three miRNAs. Expression analysis indicated that the expression of HbCAMTAs differed significantly in different tissues, and the expression was continuously up-regulated in leaves with the development of leaves and reached the highest expression level at mature or palegreen. Except for HbCAMTA2, ethylene stimulation failed to significantly induce the expression of other CAMTA genes in rubber tree while low-temperature could significantly induce the up-regulated expression of HbCAMTAs. HbCAMTA1 was constitutively expressed in all samples, and also mostly expressed in latex, leaves, roots, bark, male and female flowers, and four different leaf developmental stages (Bronzite, Changecolor, Palegreen, and Mature), which suggesting its fundamental function in rubber tree. HbCAMTA3 was expressed at the highest level in the seeds and was significantly up-regulated by low temperature stress, indicating its important role in response to low temperature stress. HbCAMTA7 was undetectably express in all investigated samples including different tissues, leaf developments, and treatments. Finally, this study would provide important information for further revealing the function of HbCAMTAs in rubber tree.

Key words: Hevea brasiliensis, transcription factor, CAMTA, gene expression

CLC Number: