Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2813-2818.DOI: 10.3969/j.issn.1000-2561.2021.10.009

• Omics & Biotechnology • Previous Articles     Next Articles

Cloning and Expression Analysis of MeLHCB4 from Cassava

DING Kaixuan1,2, ZHENG Wanru1,2, LI Linlin1,2, PAN Yueyun1,2, ZHANG Yindong1,2, GENG Mengting1,2,*(), CHEN Yinhua1,2   

  1. 1. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China
  • Received:2021-01-13 Revised:2021-03-01 Online:2021-10-25 Published:2021-11-25
  • Contact: GENG Mengting

Abstract:

In this study, the MeLHCB4 coding region of cassava chlorophyll a-binding b binding protein gene was cloned by the RT-PCR technique. The structure of the gene and the physical and chemical properties of the protein encoded by the gene were analyzed by bioinformatics, and the LHCB4 amino acid sequences of different species were compared and the evolutionary tree was constructed. The results showed that the CDs sequence of cassava MeLHCB4 was 858 BP, encoding 285 amino acids, the theoretical molecular weight of the protein was about 30.9 kDa, and the theoretical isoelectric point was 5.47. The protein was a stable hydrophilic protein, and it was predicted that the protein might be located in the nucleus and cytoplasm. The expression pattern of MeLHCB4 was detected by real-time fluorescence quantitative PCR. It was found that MeLHCB4 was mainly expressed in the leaves and stems of cassava, and was induced by hormones such as JA, SA and ACC, which was speculated to be involved in JA, SA, ACC signal pathway. After 12 hours of Xanthomonas axonopodis pv. manihotis infecting cassava leaves, the expression of MeLHCB4 increased significantly, indicating that MeLHCB4 was involved in the response process of cassava to pathogens.

Key words: Manihot esculenta, chlorophyll binding protein, MeLHCB4, gene cloning, gene expression

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