Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2806-2812.DOI: 10.3969/j.issn.1000-2561.2021.10.008

• Omics & Biotechnology • Previous Articles     Next Articles

Responses of Pineapple SVP Genes to Ethephon Stimulation

LI Yujing1,2, CHEN Zhe2,*(), HU Fuchu2, RUAN Chengcheng1,2, LUO Zhiwen2, WANG Xianghe2, FAN Hongyan2, ZHANG Zhili2,*()   

  1. 1. Institute of Life Sciences and Pharmacy, Hainan University, Haikou, Hainan 570228, China
    2. Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences / Key Laboratory of Tropical Fruit Tree Biology of Hainan Province / Haikou Investigation Station of Tropical Fruit Trees, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571100, China
  • Received:2020-12-08 Revised:2021-03-08 Online:2021-10-25 Published:2021-11-25
  • Contact: CHEN Zhe,ZHANG Zhili

Abstract:

SVP (short vegetative phase) is a type of flowering suppressor gene, which regulates the expression of flowering-related genes to affect the transition process of plants from the vegetative growth stage to the reproductive growth stage. According to the published pineapple genome information, two AcSVP genes named AcSVP1 and AcSVP2 were cloned in ‘Tainong 4’ pineapple. Analyses showed that AcSVP1 and AcSVP2 encoded 225 and 230 amino acids, respectively, and both contained MADS-box and K-box conserved domains. The proteins encoded belonged to the MADS-box gene family members. qRT-PCR analysis showed that the genes were expressed in different degrees in stem tip, stem base and leaf tissues, and after ethephon treatment the genes were mainly down-regulated to varying degrees compared with the control. Within 8 h of ethephon treatment, AcSVP1 was significantly down-regulated in the stem tips and leaves while in the stem-basal tissue which showed a trend of first down and then up-regulation, and the relative expression of AcSVP1 in the stem-basal tissues was slightly higher than that of its control after 8 h of ethephon treatment. Different from AcSVP1, AcSVP2 gene was significantly down-regulated in stem tip, stem base and leaf tissues within 8 h of ethephon treatment, and the relative expression level of AcSVP2 in the stem tip, stem base and leaf tissues was respectively about 8%, 44% and 33% of the control after 8 h of ethylene treatment. SVP was the most important type of flowering suppressor gene. And the down-regulated of AcSVP in response to ethephon stimulation indicated that AcSVP maybe play a key role in the flowering processes of pineapple in responses to ethephon signal.

Key words: Ananas comosus, SVP gene, gene cloning, ethephon

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