Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2751-2757.DOI: 10.3969/j.issn.1000-2561.2021.10.002

• Variety Breeding and Genetic Diversity of Tropical Crops • Previous Articles     Next Articles

Molecular Identity Card Establishment of Dendrobium Germplasms by TP-M13-SSR

XIAO Wenfang1,2, LI Zuo1,2,*(), CHEN Heming1,2, LYU Fubing1,2   

  1. 1. Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China
    2. Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou, Guangdong 510640, China
  • Received:2021-05-08 Revised:2021-06-28 Online:2021-10-25 Published:2021-11-25
  • Contact: LI Zuo

Abstract:

A total of 29 Dendrobium germplasms were studied with tailed primer M13 microsatellite markers (TP-M13- SSR). Analysis was made to perform the genetic diversity and establish the germplasms molecular ID. The results showed that by using 14 selected SSR markers, 191 alleles were detected and the detection rate was 79.31%-100% per primer. The polymorphism information content was 0.3877-0.9484, 0.7956 on average. Shannon’s information index was 0.8702-3.1553 and the average value was 2.1606. The genetic similarity coefficient of the germplasms ranged from 0.3037 to 0.9005, and all germplasms could be divided into 6 groups at 0.7000. Based on transferability and Shannon’s information index of the primers, more and more loci were added to distinguish all the germplasms. Finally, DoeSSR5, DoeSSR87 and DoeSSR97 at least were screened. Loci that amplified by the markers were coded to establish the unique and identifiable molecular ID of the germplasms.

Key words: Dendrobium, TP-M13-SSR, molecular identity card

CLC Number: