Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (9): 2653-2658.DOI: 10.3969/j.issn.1000-2561.2021.09.029

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Functional Analysis of Histone Acetyl-transferase CgGCN5 in Regulation of Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis

LIU Shike, AN Bang*()   

  1. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
  • Received:2020-08-19 Revised:2021-02-02 Online:2021-09-25 Published:2021-11-01
  • Contact: AN Bang

Abstract:

The anthracnose caused by Colletotrichum gloeosporioides is one of the major reasons for resulting in reduction of rubber yield in Hainan. Figuring out the molecular mechanisms of pathogenicity of C. gloeosporioides could provide basis for controlling strategy against the pathogen. The histone acetyl-transferase encoding gene CgGCN5 was identified in the genome of C. gloeosporioides. In the present study, the CgGCN5 knock-out mutant and the complementation mutant strains were constructed by homologous recombination strategy. Phenotype analysis revealed that the vegetative growth and sporulation were significantly reduced in the knock-out mutant. The pathogenicity of the mutant to rubber leaves was also significantly impaired. Further analysis suggested that the knock-out of CgGCN5 lead to the loss of penetration ability of the pathogen to cellophane. In addition, the impair of growth and virulence were restored in the complementation strain. The results suggested that CgGCN5 plays important roles in the regulation of growth and pathogenicity of C. gloeosporioides.

Key words: Colletotrichum gloeosporioides, histone acetyl-transferase, GCN5, pathogenicity

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