Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (9): 2443-2450.DOI: 10.3969/j.issn.1000-2561.2021.09.002

• Omics & Biotechnology • Previous Articles     Next Articles

Identification and Expression Analysis of profilin Gene Family in Hevea brasiliensis

DENG Zhi, LI Dejun*()   

  1. Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Haikou, Hainan 571101, China
  • Received:2020-12-16 Revised:2021-02-01 Online:2021-09-25 Published:2021-11-01
  • Contact: LI Dejun

Abstract:

Actin cytoskeleton might play an important role in laticifer wound plugging of Hevea brasiliensis. Although profilin is a vital regulator in actin dynamics, there is still no systematic study on profilin gene family in Hevea brasiliensis. In this study, six profilin genes were identified from the genome and transcriptome of Hevea brasiliensis. The six genes were analyzed in details, including gene basic characteristics, conserved protein motifs, structure features, evolutionary relationships, and expression profiles. Gene structure analyses demonstrated that the six profilin genes contained three exons and two introns. Conserved domain analyses indicated that the six profilin proteins possessed unique motifs of profilin protein, KYMVIQGE and VIRGKKG motifs. Evolutionary analyses showed that Hevea profilins were not strictly divided into vegetative and reproductive subclasses. The main secondary structures of profilin proteins were random coil and tertiary structures consist of three α helices and seven β turns. Expression analyses showed that four profilin genes were highly expressed in latex, and regulated by latex flow and potassium iodide treatment, suggesting that profilin genes might be involved in latex flow. These results would lay a foundation for further elucidating profilin roles in laticifer wound plugging and latex flow of Hevea brasiliensis.

Key words: Profilin gene family, Hevea brasiliensis, latex flow, expression analysis

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