Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (8): 2158-2166.DOI: 10.3969/j.issn.1000-2561.2021.08.006

• Omics & Biotechnology • Previous Articles     Next Articles

Cloning and Expression Analysis of Phosphate Starvation Responsive Genes, SgPHR1 and SgPHR2, in Stylosanthes guianensis

HUANG Jie1, SONG Jianling1, AN Na1, ZOU Xiaoyan1, LI Jifu1, LIU Guodao2, CHEN Zhijian1,2,*()   

  1. 1. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture & Rural Affaprs, Haikou, Hainan 571101, China
  • Received:2020-08-10 Revised:2020-09-26 Online:2021-08-25 Published:2021-09-09
  • Contact: CHEN Zhijian

Abstract:

Low phosphorus (P) stress is one of the constraints limiting crop growth and yield. PHR (phosphate starvation response) is the key factor in P signaling network, regulating plant phosphate (Pi) homeostasis. In this study, two transcription factors, SgPHR1 and SgPHR2, were cloned in stylo (Stylosanthes guianensis). The full length of SgPHR1 and SgPHR2 was 1413 bp and 849 bp, encoding 470 and 282 amino acid residues, respectively. The protein molecular weight of SgPHR1 and SgPHR2 was 51.4 kD and 30.9 kD, respectively. Both SgPHR1 and SgPHR2 belonged to the SANT family and contained MYB protein domain and CC protein domain. Both SgPHR1 and SgPHR2 included a set of potential phosphorylation sites. Subcellular localization prediction indicated that both SgPHR1 and SgPHR2 localized to the nucleus. Real-time quantitative PCR results showed that the transcript of SgPHR1 in leaves and roots was higher than that in stems, while SgPHR2 exhibited the highest expression in leaves. Furthermore, the expression of SgPHR1 and SgPHR2 was up-regulated by both -P and nitrogen (-N) deficient treatments in stylo roots. The transcription level of SgPHR1 and SgPHR2 was found to be increased during -P treatments, suggesting that SgPHR1 and SgPHR2 involved in stylo response to Pi starvation. This study would provide candidate genes for investigating the mechanism of stylo response to P deficiency.

Key words: low phosphorus stress, Stylosanthes, phosphate starvation response, gene expression, transcription factor

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