Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (6): 1750-1755.DOI: 10.3969/j.issn.1000-2561.2021.06.034

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Matrix Effects in the Determination of 5 Pesticides in Tropical Fruits by UPLC-MS/MS

ZHAO Jing1,2, HE Yan2, QIAN Bing2,3, ZHANG Yue2, HAN Bingjun2,*(), PENG Lixu4   

  1. 1. College of Plant Protection, Hainan University, Haikou, Hainan 570228, China
    2. Analysis & Testing Center, Chinese Academy of Tropical Agricultural Sciences / Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, Hainan 571101, China
    3. College of Forestry, Hainan University, Haikou, Hainan 570228, China
    4. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2020-06-10 Revised:2020-08-20 Online:2021-06-25 Published:2021-07-19
  • Contact: HAN Bingjun


The matrix effect of banana, mango, pineapple and muskmelon on the determination of fosthiazate, tebuconazole, spirotetramat, azoxystrobin and chlorantraniliprole residues in four tropical fruits was studied by UPLC- MS/MS. The results showed that in the range from 0.005 to 0.5?μg/mL, the coefficient of determination (r) was greater than 0.999. The recovery (n=5) was 76%-130% with relative standard deviations (RSDs) of 0.2%-12.9%. Fosthiazate, spirotetramat, azoxystrobin and chlorantraniliprole showed matrix enhancing effect in four tropical fruit matrixes, ranging from 97.0% to 178.8%. Tebuconazole at low concentration showed matrix inhibitory effect, ranging from 56.6% to 65.7%, the rest showed matrix enhancing effect. Among them, the matrix effect of mango in the four tropical fruits was more obvious, which was 63.0%-178.8%. Tebuconazole and spirotetramat in the five pesticides were more obvious, tebuconazole was 56.6%-129.9%, spirotetramat was 107.1%-178.8%. In actual detection, it is recommended to use matrix-matched standards for quantitative analysis for enhancing the accuracy of testing results. This experiment could provide a basis for the detection of pesticide residues in tropical fruits, and provide a reference for the reduction of matrix effect.

Key words: matrix effects, ultra performance liquid chromatography-tandem mass spectrometry, tropical fruits, pesticide residue

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