Distribution and Pathogen Detection of Cassava Mosaic Virus Disease in China

doi:10.3969/j.issn.1000-2561.2021.06.023

Abstract

Abstract:

To clarify the disease situation, symptom types and pathogens of cassava mosaic virus disease in China, 19 locations in 8 provinces were investigated in 2018-2019, and 384 narcissus samples were collected. Data analysis results showed that cassava mosaic virus disease had already occurred in China Stem transportation was the main reason for the long-distance transmission of the disease. Specific primers of Sri Lankan cassava mosaic virus (SLCMV) and Cassava common mosaic virus (CsCMV) were used to detect the 384 samples. The results showed that SLCMV and CsCMV were detected in 21.1% and 36.5% samples, respectively. 15.1% samples were mix-infected by the two viruses. The sequence similarity between the detected SLVMV and Southeast Asian SLCMV was 99.4%-99.7%, and the sequence similarity between CsCMV and Latin America was 91%-96%. It indicates that domestic cassava mosaic disease is mainly caused by these two viruses, and theCommon mosaic virus may have some sequence mutation.

Key words: Manihot esculenta, Sri Lankan cassava mosaic virus (SLCMV), Cassava common mosaic virus (CsCMV), detection, mixed infection

CLC Number:

S435.33

WANG Guofen, LI Chaoping, SHI Tao, ZHOU Sishan, LI Boxun, CAI Jimiao, LIN Zhaowei, HUANG Guixiu. Distribution and Pathogen Detection of Cassava Mosaic Virus Disease in China[J]. Chinese Journal of Tropical Crops, 2021, 42(6): 1668-1677.

Figures/Tables 7

References 27

[1]	Legg J, Somado E A, Barker I, et al. A global alliance declaring war on cassava viruses in Africa[J]. Food Security, 2014, 6(2):231-248. DOI URL
[2]	Legg J P, Fauquet C M. Cassava mosaic geminiviruses in Africa[J]. Plant Molecular Biology, 2004, 56(4):585-599. DOI URL
[3]	Thresh J M, Otim-Nape G W, Legg J P, et al. African cassava mosaic virus disease: The magnitude of the problem[J]. African Journal of Root & Tuber Crops, 1997, 2:13-19.
[4]	Wang H L, Cui X Y, Wang X W, et al. First report of Sri Lankan cassava mosaic virus infecting cassava in Cambodia[J/OL]. Plant Disease, 2015, 100(5), DOI: 10.1094/PDIS-10-15-1228-PDN. DOI
[5]	Minato N, Sok S, Chen S, et al. Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015[J/OL]. PLoS One, 2019, 14(2), DOI: 10.13140/RG.2.2.20766.69442. DOI
[6]	Tackling Cassava Mosaic Disease in Southeast AsiaZ/OL. (2020-05-20]. https://blog.ciat.cgiar.org/tack-ling-cassava-mosaic-disease-in-southeast-asia/#.
[7]	中华人民共和国农业部公告第862号《中华人民共和国进境植物检疫性有害生物名录》[EB/OL]. (2007-06-04)[2020-05-20]. http://www.moa.gov.cn/gk/tzgg_1/gg/200706/t20070604_827310.htm.
[8]	Wang D, Yao X M, Huang G X, et al. First report of Sri Lankan cassava mosaic virus infected cassava in China[J]. Plant Disease, 2018, 103(6):1437.
[9]	时涛, 王国芬, 李超萍, 等. 木薯花叶病在中国发生的首次报道[J]. 热带农业科学, 2018, 38(10):99-100.
[10]	Jacobson A L, Duffy S, Sseruwagi P, et al. Whitefly-transmitted viruses threatening cassava production in Africa[J]. Current Opinion in Virology, 2018, 33:167-176. DOI PMID
[11]	Chen C T, Ko N C, Chen M J. Electron microscopy of cassava common mosaic in Taiwan[J]. Republic of the Taiwan Sugar Research Inspectorate, 1981, 93:20-27.
[12]	谢艳, 张仲凯, 李正和, 等. 粉虱传双生病毒的TAS-ELISA及PCR快速检测[J]. 植物病理学报, 2002, 32 (2):182-186.
[13]	Fernandez E, Espinoza I, Lozano I, et al. First report of cassava common mosaic disease and Cassava common mosaic virus infecting cassava (Manihot esculenta) in Peru[J]. Plant Disease, 2017, 101(6):1066.
[14]	Uke A, Hoat T X, Quan M V, et al. First report of Sri Lankan cassava mosaic virus infecting cassava in Vietnam[J]. Plant Disease, 2018, 102(12):2669.
[15]	Lozano I, Leiva A M, Jimenez J, et al. Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene[J]. Virus Research, 2017, 241:53-61. DOI URL
[16]	时涛, 蔡吉苗, 黄贵修. 木薯花叶病和褐条病的安全性评估[J]. 热带农业科学, 2015, 35(5):23-28.
[17]	时涛, 王国芬, 李超萍, 等. 柬埔寨木薯主要病害及从中国引进品种花叶病发生情况[J]. 中国植保导刊, 2019, 39(11):91-95.
[18]	时涛, 黄贵修. 我国木薯主栽品种在乌干达发生花叶病和褐条病的调查[J]. 中国植保导刊, 2016, 36(1):73-75, 83.
[19]	薛茂富, 朱文丽, 李开绵, 等. 刚果(布)耐花叶病高产木薯品种筛选[J]. 热带作物学报, 2015, 36(10):1779-1784.
[20]	Legg J P, Lava Kumar P, Makeshkumar T, et al. Cassava virus diseases: Biology, epidemiology, and management[J]. Advances in Virus Research, 2015, 91(1):85-142.
[21]	Zhou X, Liu Y, Calvert L, et al. Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination[J]. Journal of General Virology, 1997, 78(8):2101-2111. DOI URL
[22]	Venturini M T, Araújo T D S, Abreu E, et al. Crop losses in Brazilian cassava varieties induced by the Cassava common mosaic virus[J]. Scientia Agricola, 2016, 73(6):520-524. DOI URL
[23]	李开绵, 林雄, 黄洁. 国内外木薯科研发展概况[J]. 热带农业科学, 2001(1):56-60.
[24]	Boykin L M, Sseruwagi P, Alicai T, et al. Tree lab: Portable genomics for early detection of plant viruses and pests in Sub-Saharan Africa[J]. Genes, 2019, 10(9):632. DOI URL
[25]	Otti G, Bouvaine S, Kimata B, et al. High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants[J]. Journal of Applied Microbiology, 2016, 120(5):1346-1356. DOI URL
[26]	Uke A, Khin S, Kitaura K, et al. Combination of an image-posting system and molecular diagnosis for detecting Sri Lankan cassava mosaic virus[J]. Tropical Plant Pathology, 2019, 44(3):238-243. DOI URL
[27]	Rache L, Blondin L, Flores C, et al. An optimized microsatellite scheme for assessing populations of Xanthomonas phaseoli pv. manihotis[J]. Phytopathology, 2019, 109(5):859-869. DOI URL

靶标病毒 Target	序列（5°-3°） Sequences (5°-3°)	条带大小 Sizes of products/bp	来源 References
Begomovirus	PA: TAATATTACGKGWKGV CCSC PB: TGGACYTTRCAWGGB CCTTCACA	500	[12]
Sri Lankan cassava mosaic virus (SLCMV)	AF: ATCATTTCAACTCCCG CCTCGAAG AR: CACGCCATGTACAGC ATCAATGCA	674	[8]
Sri Lankan cassava mosaic virus (SLCMV)	BF: ATTTGGATCCTATTAG ACTTGGG BR: TAATAGGATCCAAATC CATGAGAT	2700	[8]
Cassava common mosaic virus (CsCMV)	CsCMV-3269-F: GAGGCTC TTCTCTGGGAAAC CsCMV-3896-R: CTTGAGTC CAGTTTGATGTC	627	[13]

靶标病毒 Target	序列（5°-3°） Sequences (5°-3°)	条带大小 Sizes of products/bp	来源 References
Begomovirus	PA: TAATATTACGKGWKGV CCSC PB: TGGACYTTRCAWGGB CCTTCACA	500	[12]
Sri Lankan cassava mosaic virus (SLCMV)	AF: ATCATTTCAACTCCCG CCTCGAAG AR: CACGCCATGTACAGC ATCAATGCA	674	[8]
Sri Lankan cassava mosaic virus (SLCMV)	BF: ATTTGGATCCTATTAG ACTTGGG BR: TAATAGGATCCAAATC CATGAGAT	2700	[8]
Cassava common mosaic virus (CsCMV)	CsCMV-3269-F: GAGGCTC TTCTCTGGGAAAC CsCMV-3896-R: CTTGAGTC CAGTTTGATGTC	627	[13]

序号 No.	地址 Location	经纬度 Latitude and longitude	2018		2019
序号 No.	地址 Location	经纬度 Latitude and longitude	种质发病情况 Germplasm infection situation	Gs/Ts(GIR)	种质发病情况 Germplasm infection situation	Gs/Ts(GIR)
1	广东开平	22°33°21? N, 112°39°17? E	SC16、O411、O184发病	3/30(10.0%)	SC16、ZM8229、SC205等9份发病	9/18(50.0%)
2	江西抚州	28°12°30? N, 116°29°36? E	JG1301、O440、I009-1等14份发病	14/30(46.7%)	桂木薯4号、桂木薯2号、桂木薯5号等12份发病	12/188(6.4%)
3	福建三明	25°41°24? N, 117°55°17? E	热引1号、O548、O170等7份发病	7/11(63.6%)	GR911、SC205、NZ199等13份发病	13/46(28.3%)
4	广西武鸣	23°208°67? N, 108°377°463? E	SC12、SC205、新选048等12份发病	12/15(80.0%)	GR05、新选048、GR9等12份发病	12/27(44.4%)
5	广西贵港	23°208°67? N, 108°377°463? E	GR7、NZ199、SC205均未发病	0/20(0)	5-x-12、NZ199、46-13等6份发病	6/20(30.0%)
6	湖南醴陵	27°32°34? N, 113°31°18? E	-	-	47-18、32-8、C9等13份发病	13/20(65.0%)
7	云南保山	24°57°47? N, 98°52°54? E	-	4/260(1.5%)	56-2、39-2、27-4等4份发病	4/280(1.4%)
8	广西北海	21°40°47? N, 109°15°47? E	NZ199等主栽品种未发病	0/4(0)	SC205、P478、SMN03等9份发病	9/18(50.0%)
9	海南白沙	19°20°5? N, 110°15°41? E	主栽品种立民未发病	0/20(0)	014、18、PS48等11份发病	11/55(20.0%)
10	广西桂林	25°4°3? N, 110°18°101? E	-	-	NZ199、SC205、SMN03等11份发病	11/28(39.3%)
11	贵州兴义	25°11°19? N, 106°4°3? E	-	6/600(1.0%)	SMN03、44-1-1、39-2等29份发病	29/600(4.8%)
12	海南儋州宝岛新村	19°30°36? N, 109°30°20? E	E1169、E1340、F50等7份发病	7/500(1.4%)	M592、M35、M346等27份发病	27/500(5.4%)
13	广西隆安	22°987°81? N, 107°876°71? E	桂木薯3、LiMCK、GCK9等5份发病	5/200(2.5%)	桂木薯3号、SC205、SC9等28份发病	28/200(14.0%)
14	海南儋州东城	19°43°56? N, 109°34°14? E	-	3/40(7.5%)	SC9-1、SC9-2、SC9-3等4份发病	4/300(1.3%)
15	海南澄迈	19°50°55? N, 110°4°37? E	GR5、NZ199、SC205等14份发病	14/-	-	3/-
16	海南儋州沙田	19°30°35? N, 110°28°58? E	SC7、KU58、F274等9份发病	9/26(34.6%)	宝岛9-5、22、LD713等16份发病	16/-
17	海南儋州六坡	19°34°28? N, 109°30°06? E	SC16、SC8、46-14发病	3/-	-	-
18	广西崇左	22°39°7? N, 107°55°15? E	-	-	刘氏芬、CN3993、1289等28份发病	28/200(14%)
19	海南文昌	19°32°17? N, 110°45°52? E	SC10、SC205发病	2/13(15.4%)	SC10、SC205、NZ199发病	3/40(7.5%)
20	广西隆安	22°59°31? N, 107°52°47? E	-	-	农户种植NZ199、印尼细叶木薯均未发病
21	广西隆安	22°56°30? N, 107°56°22? E	-	-	农户种植NZ199未发病
合计			89/1725(5.2%)		238/2540(9.4%)

序号 No.	地址 Location	经纬度 Latitude and longitude	2018		2019
序号 No.	地址 Location	经纬度 Latitude and longitude	种质发病情况 Germplasm infection situation	Gs/Ts(GIR)	种质发病情况 Germplasm infection situation	Gs/Ts(GIR)
1	广东开平	22°33°21? N, 112°39°17? E	SC16、O411、O184发病	3/30(10.0%)	SC16、ZM8229、SC205等9份发病	9/18(50.0%)
2	江西抚州	28°12°30? N, 116°29°36? E	JG1301、O440、I009-1等14份发病	14/30(46.7%)	桂木薯4号、桂木薯2号、桂木薯5号等12份发病	12/188(6.4%)
3	福建三明	25°41°24? N, 117°55°17? E	热引1号、O548、O170等7份发病	7/11(63.6%)	GR911、SC205、NZ199等13份发病	13/46(28.3%)
4	广西武鸣	23°208°67? N, 108°377°463? E	SC12、SC205、新选048等12份发病	12/15(80.0%)	GR05、新选048、GR9等12份发病	12/27(44.4%)
5	广西贵港	23°208°67? N, 108°377°463? E	GR7、NZ199、SC205均未发病	0/20(0)	5-x-12、NZ199、46-13等6份发病	6/20(30.0%)
6	湖南醴陵	27°32°34? N, 113°31°18? E	-	-	47-18、32-8、C9等13份发病	13/20(65.0%)
7	云南保山	24°57°47? N, 98°52°54? E	-	4/260(1.5%)	56-2、39-2、27-4等4份发病	4/280(1.4%)
8	广西北海	21°40°47? N, 109°15°47? E	NZ199等主栽品种未发病	0/4(0)	SC205、P478、SMN03等9份发病	9/18(50.0%)
9	海南白沙	19°20°5? N, 110°15°41? E	主栽品种立民未发病	0/20(0)	014、18、PS48等11份发病	11/55(20.0%)
10	广西桂林	25°4°3? N, 110°18°101? E	-	-	NZ199、SC205、SMN03等11份发病	11/28(39.3%)
11	贵州兴义	25°11°19? N, 106°4°3? E	-	6/600(1.0%)	SMN03、44-1-1、39-2等29份发病	29/600(4.8%)
12	海南儋州宝岛新村	19°30°36? N, 109°30°20? E	E1169、E1340、F50等7份发病	7/500(1.4%)	M592、M35、M346等27份发病	27/500(5.4%)
13	广西隆安	22°987°81? N, 107°876°71? E	桂木薯3、LiMCK、GCK9等5份发病	5/200(2.5%)	桂木薯3号、SC205、SC9等28份发病	28/200(14.0%)
14	海南儋州东城	19°43°56? N, 109°34°14? E	-	3/40(7.5%)	SC9-1、SC9-2、SC9-3等4份发病	4/300(1.3%)
15	海南澄迈	19°50°55? N, 110°4°37? E	GR5、NZ199、SC205等14份发病	14/-	-	3/-
16	海南儋州沙田	19°30°35? N, 110°28°58? E	SC7、KU58、F274等9份发病	9/26(34.6%)	宝岛9-5、22、LD713等16份发病	16/-
17	海南儋州六坡	19°34°28? N, 109°30°06? E	SC16、SC8、46-14发病	3/-	-	-
18	广西崇左	22°39°7? N, 107°55°15? E	-	-	刘氏芬、CN3993、1289等28份发病	28/200(14%)
19	海南文昌	19°32°17? N, 110°45°52? E	SC10、SC205发病	2/13(15.4%)	SC10、SC205、NZ199发病	3/40(7.5%)
20	广西隆安	22°59°31? N, 107°52°47? E	-	-	农户种植NZ199、印尼细叶木薯均未发病
21	广西隆安	22°56°30? N, 107°56°22? E	-	-	农户种植NZ199未发病
合计			89/1725(5.2%)		238/2540(9.4%)

侵染类型 Infection type	检测样品数 Sample number	阳性样品数 Positive samples number	比例 Ratio/%
SLCMV	384	81	21.1
CsCMV	179	141	78.8
SLCMV+CsCMV	179	59	33.0