Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (6): 1549-1556.DOI: 10.3969/j.issn.1000-2561.2021.06.006

• Germplasm Resources, Genetics & Breeding • Previous Articles     Next Articles

Construction of cpSSR Fingerprints and Genetic Diversity Analysis of 104 Sweetpotato Varieties

WANG Chong1,2, WANG Lianjun1, YANG Xinsun1, LEI Jian1, CHAI Shasha1, ZHANG Wenying2, JIAO Chunhai3,**(), TIAN Xiaohai2,**()   

  1. 1. Hubei Key Laboratory of Food Crops Germplasm and Genetic Improvement / Institute of Food Crops, Hubei Academy of Agricultural Sciences / Hubei Sweetpotato Engineering and Technology Research Centre, Wuhan, Hubei 430064, China
    2. College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China
    3. Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064, China
  • Received:2020-06-10 Revised:2020-08-17 Online:2021-06-25 Published:2021-07-19
  • Contact: JIAO Chunhai,TIAN Xiaohai

Abstract:

Based on the sweetpotato chloroplast genome, this study developed cpSSR molecular markers and analyzed the genetic diversity of 104 cultivars and landraces sweetpotato. A fingerprint map was constructed to provide references for the protection identification and genetic improvement of sweet potato resources. 11 pairs of cpSSR markers were used in this study. A total of 58 bands were amplified from 104 sweetpotato materials, with 47 polymorphic bands. The average number of bands amplified by a single primer was 5.27, and the average polymorphism percentage was 81.03%. Based on the amplification results of the primers in the sweet potato material, the fingerprints of 104 sweet potato varieties were constructed. Cluster analysis was performed on the amplification results of 104 sweetpotato varieties. The genetic distance between each variety was 0.0386 to 5.2723, and the average genetic distance was 0.2201. When the genetic coefficient was 0.74, the 104 sweet potato varieties were divided into 5 groups.

Key words: sweetpotato, cpSSR marker, genetic diversity, fingerprinting

CLC Number: