Differential Expression Analysis of Genes Related to Starch and Sucrose Metabolism Pathway in Different Developmental Stages of Dragon Fruit Pulp Based on Transcriptome
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (6): 1520-1530.DOI: 10.3969/j.issn.1000-2561.2021.06.003

• Omics & Biotechnology • Previous Articles     Next Articles

Differential Expression Analysis of Genes Related to Starch and Sucrose Metabolism Pathway in Different Developmental Stages of Dragon Fruit Pulp Based on Transcriptome

YANG Yunliang, LI Jianxun(), MA Genong   

  1. Cotton Research Institute, Shanxi Agricultural University, Yuncheng, Shanxi 044000, China
  • Received:2020-07-02 Revised:2020-09-04 Online:2021-06-25 Published:2021-07-19
  • Contact: LI Jianxun

Abstract:

In this study, Illumina Novaseq 6000 sequencing was used to sequence the samples from three stages of dragon fruit pulp (young fruit stage, color conversion stage and mature stage). The unigenes obtained was annotated in seven databases. A total of 3617 unigenes were successfully annotated in all databases. Five enzyme genes related to starch and sucrose metabolism pathway were further screened and verified by quantitative Real-time PCR (qPCR). The results showed that 115 193 transcripts and 47 855 transcripts were obtained by transcriptome sequencing and unigene N50 was 2000. The results of GO functional enrichment analysis showed that 18 582 unigenes were divided into three categories: biological process, cell composition and molecular function in GO functional annotation. The results of KOG functional classification showed that 6203 unigenes were divided into 25 group, in KOG annotation, of which post-translational modification, protein turnover and chaperone contained the most unigenes. The results of KEGG metabolic pathway annotation showed that 6554 unigenes got functional annotation, and there were 119 metabolic pathways, among which carbohydrate metabolism accounted for the highest proportion. Five key enzyme genes involved in starch and sucrose metabolism and synthesis pathway, such asUGP2, glgC, glgA, Cluster-12747.5831 and Cluster-12747.16617, were screened. The expression level of qPCR was consistent with that of transcriptome sequencing.

Key words: dragon fruit, pulp, transcriptome sequencing, starch, sucrose

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