Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (6): 1514-1519.DOI: 10.3969/j.issn.1000-2561.2021.06.002

• Omics & Biotechnology • Previous Articles     Next Articles

Subcellular Localization Analysis and Responses to Exogenous ABA and SA in Longan Callus of DCL4

ZAHNG Xueying, LIANG Zihao, CHEN Xiaohui, ZHANG Shuting, LAI Zhongxiong(), LIN Yuling()   

  1. Institute of Horticultural Biotechnology, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2020-07-23 Revised:2020-09-03 Online:2021-06-25 Published:2021-07-19
  • Contact: LAI Zhongxiong,LIN Yuling

Abstract:

This study carried out amino acid sequence alignment, phylogenetic tree analysis, subcellular localization verification and real-time qPCR analysis to study the function of DlDCL4 gene of DlDCL family in longan. It was found that the similarity of the amino acid sequence of DCL4 was more than 50% in the selected species. In the evolutionary relationship, DlDCL4 and navel orange (Citrus sinensis) were closely related. Under the observation of laser confocal microscopy, the onion cell nucleus emitted green fluorescence, and other parts did not emit fluorescence, so the longan DCL4 protein was localized in the nucleus. In 24 h, 100 μmol/L exogenous ABA hormone had a significant down-regulation effect on DlDCL4. SA had a significant up-regulation effect on its expression, and the effect was higher than other time treatment at 8 h, which suggested thatDlDCL4 had different responses to different hormones and this effect would also be affected by different treatment durations.

Key words: longan, DCL4 gene, subcellular localization, hormone treatment

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