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Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (6): 1505-1513.DOI: 10.3969/j.issn.1000-2561.2021.06.001

• Omics & Biotechnology •     Next Articles

Identification and Expression Analysis of Genes Related to Longan miRNA Synthesis

HUO Wen, CHEN Xiaohui, XU Xiaoping, SHEN Xu, LI Xiaofei, LAI Zhongxiong()   

  1. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2020-06-23 Revised:2020-09-15 Online:2021-06-25 Published:2021-07-19
  • Contact: LAI Zhongxiong
  • About author:LAI Zhongxiong,E-mail: Laizx01@163.com


In order to understand the biological functions of genes related to the longan miRNA synthesis pathway, we identified and analyzed the miRNA synthesis pathway-related genes in the third-generation genomic data of longan. And longan transcriptomes in the early stage of somatic embryogenesis (SE) was used for miRNA synthesis pathway-related genes expression analysis. A total of 12 genes related to longan miRNA synthesis pathway were identified, namely DlDDL1, DlDDL2, DlDDL3, DlDCL1, DlSE, DlHYL1a, DlHYL1b, DlHYL1c, DlCBP20, DlCBP80, DlHEN1, DlHASTY. The number of amino acids encoded by these genes was mostly between 400-1000 aa, and the molecular weight of the protein was 23.92-220.25 kDa. Most proteins were acidic proteins, all the proteins were unstable proteins. Except DlHASTY which is hydrophobic protein, the others were hydrophilic proteins. Gene structure analysis showed that the number of introns of each member varied greatly from 2 to 23. All members contained conservative domains, and the number difference varied from 1 to 7. Analysis of cis-acting elements in the promoter regions of gens related to miRNA synthesis pathway showed that a large number of light-responsive elements and hormone-responsive elements were presented in the promoter region of these genes. Protein interaction prediction analysis showed that the longan miRNA synthesis pathway-related genes had strong interaction relationship. Analysis of expression during the early stage of SE in longan showed that each gene had a high level of transcription at the EC stage. This study indicates that the genes related to the longan miRNA synthesis pathway may participate in the early development of longan SE development and play an important role in the EC stage, as well as responding to multiple hormone responses and abiotic stresses. Moreover, it is suggested that miRNA synthesis pathway-related genes might have diversity and differences in biological functions.

Key words: longan, miRNA synthesis pathway-related genes, bioinformatics analysis, qPCR analysis

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