Transcriptome Analysis of Flowering Chinese Cabbage (<i>Brassica campestris</i> L. ssp. <i>Chinensis</i> (L.) var. <i>utilis </i>Tsen et Lee) Infected by Soft Rot Disease
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (5): 1433-1439.DOI: 10.3969/j.issn.1000-2561.2021.05.032

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Transcriptome Analysis of Flowering Chinese Cabbage (Brassica campestris L. ssp. Chinensis (L.) var. utilis Tsen et Lee) Infected by Soft Rot Disease

ZENG Xiaoling1, ZHAO Ruili1, ZHONG Kaiqin1, ZHU Chaohui1, XIE Xinxin1, WEN Qingfang2,*()   

  1. 1. Fuzhou Institute of Vegetable Science, Fuzhou, Fujian 350111, China
    2. Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
  • Received:2020-04-20 Revised:2020-07-03 Online:2021-05-26 Published:2021-05-25
  • Contact: WEN Qingfang

Abstract:

To explore the functional gene information in flowering Chinese cabbage (Brassica campestris L. ssp. Chinensis (L.) var. utilis Tsen et Lee) inoculated by soft rot disease, the transcriptome of flowering Chinese cabbage was sequenced by BGISEQ-500 high-throughput sequencing technology. The results showed that average obtained 47.27 M clean reads and the Q20 base was more than 95%. A total of 36 760 Unigene transcripts were detected, of which 35 327 were known and 1 433 were predicted. A total of 19 549 new transcripts were detected, the length of which was mainly between 300 and 2000 nt. Functional annotation results showed that 32 047 Unigene were annotated in NR database, among which Unigenes were annotated most on Brassica rapa ssp. Pekinensis. GO function was annotated with 12 588 Unigene. Kegg database was annotated with 18 583 Unigene, involving 137 metabolic pathways. A total of 21 776 differential expression genes (DEGs) were obtained, including 15 007 DEGs, 6 137 up-regulated genes and 8 870 down- regulated genes between the control and premorbid groups. There were 13 118 DEGs, 10 278 up-regulated genes and 2840 down-regulated genes between pre-onset and mid-onset. There were 11 293 DEGs, 1 790 up-regulated genes and 9503 down-regulated genes. Analysis of the DEGs Ven map showed that 5 110 genes were different in each group. Functional analysis of DEGs showed that the differentially expressed genes were involved in various life activities, such as ubiquitin degradation pathway, peroxisome pathway, photosynthetic carbon fixation pathway, glycolytic process pathway and so on. The results of this study would lay a foundation for further studies on the genome and molecular biology of resistance to soft rot of flowering Chinese cabbage.

Key words: flowering Chinese cabbage, soft rot, transcriptome, differentially expressed genes

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