Effects Evaluation of Different Entomopathogenic Nematode Strains Against Aethina tumida (Coleoptera: Nitidulidae)

doi:10.3969/j.issn.1000-2561.2021.05.028

Abstract

Abstract:

This study aims to evaluate the pathogenicity of commercially available entomopathogenic nematodes against Aethina tumida larvae and pupa, thus develop a new agricultural method to control A. tumida beetle. The pathogenicity of 5 strains of entomopathogenic nematodes (EPNs) against wandering larvae and pupae of A. tumida were determined by the dipping and sand bioassays in the laboratory, respectively. In addition, the influence of different application time and dosage on the virulence of Steinernema carpocapsae All to A. tumida larvae was tested by sand bioassays under laboratory condition. The dipping bioassay results showed that the pathogenicity of 5 EPNs strains against A. tumida larvae was different under laboratory condition. The infection incubation of S. carpocapsae All against A. tumida larvae was the highest. The corrected mortality rate of A. tumida larvae infected by S. carpocapsae All were 67.50%±0.05% and 2.36%±3.14% after 4 d and 12 d, respectively, which was higher than that of other EPNs. The sand bioassay result demonstrated a significant treatment effect on A. tumida mortality when compared to the control. The S. carpocapsae All provided excellent control with 100% mortality of A. tumida larvae being obtained, which showed significantly higher mortality than A. tumida larvae exposed to any other nematode strains. Sequential applications of the nematodes following larvae entering sand also provided excellent control before 14 d application. The optimal control effect would be achieved when the ratio of S. carpocapsae All to A. tumida was more than 213∶1. So entomopathogenic nematodes S. carpocapsae All has a potential to control A. tumida larvae and pupae, it could be recommended for use in apiary where A. tumida occurred.

Key words: entomopathogenic nematodes, Steinernema carpocapsae, Aethina tumida, pathogenicity, bioassay

CLC Number:

S476.15
S895

HAN Wensu, WANG Zeru, LIU Yu, GAO Jinglin, ZHAO Dongxiang, ZHONG Yihai, ZHAO Shan. Effects Evaluation of Different Entomopathogenic Nematode Strains Against Aethina tumida (Coleoptera: Nitidulidae)[J]. Chinese Journal of Tropical Crops, 2021, 42(5): 1402-1408.

Figures/Tables 5

Fig. 1 Effect of direct exposure of different strains of nematodes on A. tumida larve All: S. carpocapsae All sponge adsorbent; All(P): S. carpocapsae All powder; SN(P): S. feltiae SF-SN powder; X-7(P): S. longicaudum X-7 powder; JY-90: S. feltiae JY-90 sponge adsorbent; LN2: H. indian LN2 sponge adsorbent. Different lowercase letters indicate significant difference among different treatments (P<0.05).

Fig. 2 S. carpocapsae All parasitizing A. tumida larvae under super depth of field threedimensions microscope In the black circles are S. carpocapsae All. Magnification: 100×

Fig. 3 Effect of indirect exposure of different strains of nematodes on A. tumida larvae All: S. carpocapsae All sponge adsorbent; All(P): S. carpocapsae All powder; SN(P): S. feltiae SF-SN powder; X-7(P): S. longicaudum X-7 powder; JY-90: S. feltiae JY-90 sponge adsorbent; LN2: H. indian LN2 sponge adsorbent. Different lowercase letters indicate significant difference among different treatments (P<0.05).

Fig. 4 Effect of different application time of S. carpocapsae All on A. tumida larvae All: S. carpocapsae All powder; * indicates significant difference between the treatment and CK (P<0.05, independent-sample t test).

Fig. 5 Effect of different dosages of S. carpocapsae All on A. tumida larvae The test nematode is S. carpocapsae All powder; * indicates significant difference among different treatments (Tukey’s HSD test, P<0.05).

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