Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (5): 1402-1408.DOI: 10.3969/j.issn.1000-2561.2021.05.028

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Effects Evaluation of Different Entomopathogenic Nematode Strains Against Aethina tumida (Coleoptera: Nitidulidae)

HAN Wensu1,3, WANG Zeru2, LIU Yu2, GAO Jinglin1,3,*(), ZHAO Dongxiang1,3, ZHONG Yihai1,3, ZHAO Shan1,3   

  1. 1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    2. Environment and Plant Protection College, Hainan University, Haikou, Hainan 570228, China
    3. Bee Industry Technology Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2020-05-06 Revised:2020-07-21 Online:2021-05-26 Published:2021-05-25
  • Contact: GAO Jinglin

Abstract:

This study aims to evaluate the pathogenicity of commercially available entomopathogenic nematodes against Aethina tumida larvae and pupa, thus develop a new agricultural method to control A. tumida beetle. The pathogenicity of 5 strains of entomopathogenic nematodes (EPNs) against wandering larvae and pupae of A. tumida were determined by the dipping and sand bioassays in the laboratory, respectively. In addition, the influence of different application time and dosage on the virulence of Steinernema carpocapsae All to A. tumida larvae was tested by sand bioassays under laboratory condition. The dipping bioassay results showed that the pathogenicity of 5 EPNs strains against A. tumida larvae was different under laboratory condition. The infection incubation of S. carpocapsae All against A. tumida larvae was the highest. The corrected mortality rate of A. tumida larvae infected by S. carpocapsae All were 67.50%±0.05% and 2.36%±3.14% after 4 d and 12 d, respectively, which was higher than that of other EPNs. The sand bioassay result demonstrated a significant treatment effect on A. tumida mortality when compared to the control. The S. carpocapsae All provided excellent control with 100% mortality of A. tumida larvae being obtained, which showed significantly higher mortality than A. tumida larvae exposed to any other nematode strains. Sequential applications of the nematodes following larvae entering sand also provided excellent control before 14 d application. The optimal control effect would be achieved when the ratio of S. carpocapsae All to A. tumida was more than 213∶1. So entomopathogenic nematodes S. carpocapsae All has a potential to control A. tumida larvae and pupae, it could be recommended for use in apiary where A. tumida occurred.

Key words: entomopathogenic nematodes, Steinernema carpocapsae, Aethina tumida, pathogenicity, bioassay

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