Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (5): 1245-1251.DOI: 10.3969/j.issn.1000-2561.2021.05.007

• Omics & Biotechnology • Previous Articles     Next Articles

Cloning and Expression Analysis of Sugarcane Allene Oxide Cyclase Gene (ScAOC)

XUE Yaowei, CHEN Lilan, WANG Yaru, GAO Sanji, WANG Jinda*()   

  1. National Sugarcane Engineering Technology Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 351102, China
  • Received:2020-05-19 Revised:2020-07-03 Online:2021-05-26 Published:2021-05-25
  • Contact: WANG Jinda

Abstract:

Allene oxide cyclase (AOC) is a key enzyme in the synthesis pathway of jasmonic acid and plays an important role in plant defense reactions. In this study, an AOC gene was cloned based on the sugarcane transcriptome database and named ScAOC. Biological analysis, tissue-specific analysis, and expression analysis under stress were conducted to understand the preliminary function of the gene. The open reading frame (ORF) of the ScAOC gene in sugarcane was 744 bp, encoding 244 amino acids, and its protein molecular mass was 26.37 kDa. Bioinformatics analysis showed that the ScAOC protein was an unstable basic hydrophilic protein and contained a conserved domain of the PLN02343 domain of Allene_ox_cyc Superfamily. Constructing a phylogenetic tree showed that the ScAOC protein has a high homology with sorghum protein. Fluorescence quantitative PCR showed that the ScAOC gene was expressed in the roots, stems and leaves of sugarcane, and the expression levels from high to low were leaves, roots and stems. Under the stress of PEG, NaCl and exogenous MeJA, the relative expression of ScAOC gene increased first and then decreased, and ABA had a certain inhibitory effect on the expression of ScAOC. Under the stress of pathogenic bacteria and armyworm feeding, the relative expression of ScAOC gene increased significantly. The results showed that ScAOC maight be involved in the defense response of sugarcane to biological or abiotic stress.

Key words: sugarcane, allene oxide cyclase, jasmonic acid, bioinformatics analysis

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