Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (4): 1127-1135.DOI: 10.3969/j.issn.1000-2561.2021.04.032

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Macroporous Resin Purification Process and Component Analysis of Pithecellobium clypearia Polyphenols

LI Zhichao1, FU Yongmei3, ZHANG Shu1,2, CHEN Xianglan1, XIE Zhenhui1, DENG Hong1,2,*()   

  1. 1. Key Laboratory of New Drug Dosage Form, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
    2. Guangdong Provincial Precision Medicine Drug Delivery Preparation Engineering Technology Research Center, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
    3. Sinopharm Group Guangdong Global Pharmaceutical Co., Ltd., Foshan, Guangdong 528000, China
  • Received:2020-06-20 Revised:2020-07-06 Online:2021-04-25 Published:2021-04-25
  • Contact: DENG Hong

Abstract:

The aim of the study was to establish and optimize the purification process of Pithecellobium clypearia polyphenols and qualitatively analyze its components. In this study, the crude polyphenol extract of P. clypearia was used as the raw material, and the optimal macroporous resin was screened based on the adsorption and desorption effects. Taking adsorption, desorption and purity as the evaluation indexes, the influence of various technological parameters on the purification of polyphenols by AB-8 resin was investigated. Finally, the ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) method was used for qualitative analysis of polyphenol composition. AB-8 resin was preferred, the crude extract was loaded with 10 BV at a mass concentration of 3 mg/mL and a flow rate of 1 mL/min (column volume: 1 BV=10 mL), after washing with water, eluted with 60% ethanol 5 BV at a flow rate of 1 mL/min. Under this process condition, the purity of polyphenol reached 58.64%. UPLC-Q-TOF-MS qualitatively analyzed 14 components of purified. The purification process of the established P. clypearia polyphenols was stable and feasible, and the qualitative analysis of its components was realized, which would provide a basis for further research.

Key words: Pithecellobium clypearia polyphenol, purification process, macroporous resin, Ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS)

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