Extraction of Camellia Oil from Wuyi Shuixian Tea by Ethanol and Quality Analysis

doi:10.3969/j.issn.1000-2561.2021.04.031

Abstract

Abstract:

The extraction of camellia oil of Wuyi Shuixian tea was carried out by aqueous ethanol. The effects of extraction temperature, ratio of material to liquid, concentration of ethanol and pH on the extraction rate were investigated by the single factor experiment. The orthogonal experiment design was used to optimize the extraction conditions. The results showed that the best extraction conditions were ethanol concentration 30% (V/V), material to liquid ratio 1∶7, pH 9, extraction temperature 60 ℃, under which the extraction rate of camellia oil was 93.61%. 18 kinds of fatty acids were detected in the extracted camellia oil from Wuyi Shuixian tea, mainly oleic acid (51.77%), linoleic acid (23.14%), ΣSFA∶ΣMUFA∶ΣPUFA = 1∶2.64∶1.27, and all physicochemical indexes measured meet the national standards. The results show that camellia oil from Wuyi Shuixian tea is a kind of plant edible oil with high nutritional value. The conclusion could provide theoretical basis for the extraction and quality evaluation of tea seed oil in Wuyishan tea production area.

Key words: Wuyi Shuixian tea seed, tea camellia seed oil, aqueous ethanol methodology, extraction, quality

CLC Number:

TS224.2

YU Lizhi, LIU Qilin, ZHANG Tingting, FU Chunjin. Extraction of Camellia Oil from Wuyi Shuixian Tea by Ethanol and Quality Analysis[J]. Chinese Journal of Tropical Crops, 2021, 42(4): 1120-1126.

Figures/Tables 10

References 24

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组成成分 Components	含量 Percentage/%
纯仁率	65.77±2.53
粗脂肪	25.76±3.11
粗蛋白	13.12±1.03
含水率	9.34±2.00
灰分	6.60±0.93
淀粉	19.53±1.89
茶皂	12.01±1.17

组成成分 Components	含量 Percentage/%
纯仁率	65.77±2.53
粗脂肪	25.76±3.11
粗蛋白	13.12±1.03
含水率	9.34±2.00
灰分	6.60±0.93
淀粉	19.53±1.89
茶皂	12.01±1.17

实验号 No.	因素Factors				清油得率 Yield/%
实验号 No.	A	B	C	D	清油得率 Yield/%
1	1	1	1	1	60.47
2	1	2	2	2	92.74
3	1	3	3	3	82.90
4	2	1	2	3	70.15
5	2	2	3	1	84.28
6	2	3	1	2	89.76
7	3	1	3	2	72.88
8	3	2	1	3	80.96
9	3	3	2	1	82.34
k₁	78.703	67.833	77.063	75.697
k₂	81.397	85.993	81.743	85.127
k₃	78.727	85.000	80.020	78.003
R	2.69	18.16	4.68	9.43

实验号 No.	因素Factors				清油得率 Yield/%
实验号 No.	A	B	C	D	清油得率 Yield/%
1	1	1	1	1	60.47
2	1	2	2	2	92.74
3	1	3	3	3	82.90
4	2	1	2	3	70.15
5	2	2	3	1	84.28
6	2	3	1	2	89.76
7	3	1	3	2	72.88
8	3	2	1	3	80.96
9	3	3	2	1	82.34
k₁	78.703	67.833	77.063	75.697
k₂	81.397	85.993	81.743	85.127
k₃	78.727	85.000	80.020	78.003
R	2.69	18.16	4.68	9.43

因素 Factors	平方和 Sum of squares	自由度 df	F值 F-value	F_0.05(2,2)	F_0.01(2,2)	显著性 Significance
A	14.383	2	7.09	19	99
B	625.467	2	308.11	19	99	**
C	33.614	2	16.56	19	99
D	144.987	2	71.42	19	99	*
误差	2.03	2