Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (4): 909-919.DOI: 10.3969/j.issn.1000-2561.2021.04.001

• Omics & Biotechnology •     Next Articles

Identification and Functional Analysis of 4CL Gene Family During Early Somatic Embryogenesis in Dimocarpus longan Lour

HONG Pingjing, XU Xiaoping, WANG Jingyu, CHEN Xiaohui, SHEN Xu, LIN Yuling, LAI Zhongxiong*()   

  1. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2020-04-22 Revised:2020-05-11 Online:2021-04-25 Published:2021-04-25
  • Contact: LAI Zhongxiong

Abstract:

To understand the molecular characteristics and biological functions of the 4-coumarate: coenzyme A ligase (Dl4CL) gene family in Dimocarpus longan Lour., bioinformatics analysis was used to identify the members of the 4CL gene family of longan, to analyse the protein domain and characteristics, molecular evolutionary tree, expression patterns in somatic embryogenesis and tissue-organ, and to predict the possible interaction of miRNAs. There were 43 members of the Dl4CL gene family, which could be divided into six subfamilies. The basic physical and chemical properties of different subfamilies including isoelectric point, relative molecular weight, amino acid number and signal peptide were different. Dl4CL proteins belonged to the non-secretory pathway, there were multiple conserved motifs. The structural characteristics of the genes were related to the kinship of family members in the evolutionary tree. The Dl4CL promoter sequence contained a large number of light responses elements, anaerobic induction response elements, and MYB binding sites, which suggesting that the Dl4CL gene family may be involved in the biosynthesis of flavonoids and the accumulation of pigments during the growth and development of longan. Dl4CL gene family might be involved in different somatic embryogenesis process and tissue-organ morphogenesis. A total of 10 Dl4CL members were likely to be regulated by miRNA, and different members were targeted by different miRNA, it was speculated that Dl4CL may interact with miRNA regulation process to participate in somatic embryogenesis, response to environmental stress and other biological processes. This study shows that Dl4CL might not only participate in lignin synthesis, but also participate in a variety of biological metabolic pathways, such as pigment synthesis, tissue-organ specific expression, showing the complexity of its biological functions.

Key words: longan, 4CL gene family, gene identification, functional analysis

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