Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (3): 888-896.DOI: 10.3969/j.issn.1000-2561.2021.03.039

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Comparison of Total Phenol Content and Antioxidant Activity from Different Mulberry Leaves

MA Feiyue, GENG Ju, QIAO Jian, SHUAI Xixiang, ZHANG Ming, DU Liqing*()   

  1. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture & Rural Affairs, Zhanjiang, Guangdong 524091, China
  • Received:2020-04-24 Revised:2020-05-26 Online:2021-03-25 Published:2021-03-25
  • Contact: DU Liqing


In order to compare the contents of total phenol in different mulberry leaves and the different application, single-factor experiments and a four-variable, three-level orthogonal array design were performed to study on the extraction of total phenols from 40 mulberry leaves. The optimal conditions were: temperature 65 ℃, time 30 min, solid/liquid ratio 1∶45 (g/mL), and ethanol concentration 60%. The effect of extraction condition were temperature > time > solid/ liquid ratio > ethanol concentration. Under the optimal conditions, the highest content of total phenol in ‘Tiaosang 5’ mulberry leaves was (26.35 ± 0.29) mg/g. The least content of total phenol in ‘Diansang’ mulberry leaves was (20.44 ± 0.15) mg/g. Furthermore, the most antioxidant activity of the extracts from ‘Tiaosang 5’ mulberry leaves and lowest one from ‘Diansang’ mulberry leaves was (77.64 ± 0.34) mg/L and (210.30 ± 0.19) mg/L (IC50), respectively, according to the DPPH radical-scavenging assay. According to the FRAP, TEAC of ‘Tiaosang 5’ mulberry leaves and ‘Diansang’ mulberry leaves was (2.58 ± 0.11) mmol/g and (0.73 ± 0.04) mmol/g, respectively. The total phenol content was positively correlated with the capacity of DPPH radical scavenging. Choosing varieties with high total phenol content in mulberry leaves could improve the economic values of mulberry.

Key words: different mulberry leaves, total phenol, antioxidant activity

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