Antioxidant and Bacteriostasis Activity of Flavanoid from Mallotus oblongifolius by Different Extraction Methods

doi:10.3969/j.issn.1000-2561.2021.03.033

Abstract

Abstract:

The aim was to select the suitable method for extracting the polyphenols from Mallotus oblongifolius. The polyphenols were extracted by hot water extraction, organic solvent extraction and cellulase-assisted extraction. The antioxidant activity of the extract density was evaluated by scavenging DPPH, ABTS, hydroxyl, superoxide anion radical and reducing force methods. The antibacterial activity of the polyphenol extracts was determined by filter paper diffusion method and double dilution methods. The yield of polyphenols by the hot water extraction method, organic solvent extraction method and cellulase-assisted extraction method was (10.31±0.42)%, (9.79±0.38)%, and (10.88± 0.52)%, respectively, and the content was (44.72±2.26)%, (39.54±1.78)%, and (47.07±2.52)%. The polyphenol extracts extracted by the three methods had strong antioxidant properties. The cellulose-assisted extraction of the polyphenols from M. oblongifolius (CFC) had the strongest ability to scavenge DPPH radical, and its half inhibitory concentration (IC₅₀) was (0.0034±0.0002) mg/mL. The hot water-extracted polyphenols from M. oblongifolius (WFC) had the strongest ability to scavenge ABTS radical, hydroxyl radical, and superoxide anion radical, and the IC₅₀ values was (0.066±0.004), (0.069±0.004), (0.127±0.009) mg/mL. The polyphenol extracts extracted by the three methods had a good inhibitory effect on four bacteria. Among them, WFC had a good inhibitory effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aerugino, the inhibitory zone diameter was (12.34±1.01), (12.16±0.95), (2.12±0.15), (6.12±0.36)mm, and the minimum inhibitory concentration (MIC) was 3.13, 3.13, 12.50, 6.25 mg/mL. Comprehensive consideration, the hot water extraction method is more suitable for the extraction of polyphenols from M. oblongifolius.

Key words: Mallotus oblongifolius, flavonoids, extraction, antioxidant activity, antibacterial

CLC Number:

S326

DUAN Zhouwei, LI Peng, HE Ai, WANG Shiping, XIE Hui. Antioxidant and Bacteriostasis Activity of Flavanoid from Mallotus oblongifolius by Different Extraction Methods[J]. Chinese Journal of Tropical Crops, 2021, 42(3): 847-853.

Figures/Tables 8

Tab. 1 Yield and purity of polyphenols extracted from M. oblongifolius by different methods

样品 Sample	得率 Yield/%	多酚含量 Polyphenol content/%
WFC	10.31±0.42^ab	44.72±2.26^ab
OFC	9.79±0.38^b	39.54±1.78^b
CFC	10.88±0.52^a	47.07±2.52^a

Fig. 1 Scavenging effects of polyphenols extracted from M. oblongifolius by different methods on DPPH radical

Fig. 2 Scavenging effects of polyphenols extracted from M. oblongifolius by different methods on ABTS radical

Fig. 3 Scavenging effects of polyphenols extracted from M. oblongifolius by different methods on hydroxyl radical

Fig. 4 Scavenging effects of polyphenols extracted from M. oblongifolius by different methods on superoxide anion radical

Fig. 5 Effects of polyphenols extracted extracted from M. oblongifolius by different methods on reduction power

Tab. 1 Inhibitory zone diameter of polyphenols extracted from M. oblongifoliusby different methods on test bacteria

提取物 Extracts	抑菌圈直径Diameter of the inhibition zone/mm
提取物 Extracts	大肠杆菌E. coli	金黄色葡萄球菌S. aureus	枯草芽孢杆菌B. subtilis	绿脓杆菌P. aerugino
WFC	12.34±1.01^a	12.16±0.95^a	2.12±0.15^a	6.12±0.36^a
OFC	10.83±0.62^b	10.65±0.86^b	1.96±0.13^b	5.57±0.31^b
CFC	11.52±0.82^ab	11.32±0.92^ab	2.06±0.15^ab	5.96±0.45^ab

Tab. 3 MIC of polyphenols extracted from M. oblongifolius by different methods on test bacteria

提取物 Extracts	MIC/(mg?mL^?1)
提取物 Extracts	大肠杆菌 E. coli	金黄色葡萄球菌 S. aureus	枯草芽孢杆菌 B. subtilis	绿脓杆菌 P. aerugino
WFC	3.13	3.13	12.50	6.25
OFC	6.25	6.25	25.00	12.50
CFC	3.13	3.13	12.50	6.25

References 31

[1]	顾文亮, 谭乐和, 郝朝运, 等. 鹧鸪茶的研究进展与开发利用现状[J]. 热带农业科学, 2015,35(2):28-35.
[2]	朱学雷, 安树青, 张立新, 等. 海南五指山热带山地雨林主要种群结构特征分析[J]. 应用生态学报, 1999,10(6):641-644.
[3]	宋捷, 张志远. 山苦茶的化学成分和药理作用研究进展[J]. 中国医院药学杂志, 2016,36(2):152-156.
[4]	Wei K, Li W, Koike K, et al. Two New Galloylgluco sides from the leaves of Mallotus furetianus[J]. Chemical & Pharmaceutical Bulletin, 2004,52(6):776. DOI URL PMID
[5]	Liu Y L, Wang L Q, Wu H T, et al. Comparison of anti-atherosclerotic effects of two different extracts from leaves of Mallotus furetianus[J]. Asian Pacific Journal of Tropical Medicine, 2011,4(11):878-882. URL PMID
[6]	瞿兆蕙, 吴淑敏, 杜磊, 等. 海南不同地区鹧鸪茶挥发性成分HS-SPME-GC/MS分析与评价[J]. 食品研究与开发, 2019,40(22):151-158.
[7]	李晓彤, 魏静, 谷满屯, 等. 鹧鸪茶提取物抗氧化活性研究与抗氧化特征成分的HPLC-MS/MS分析[J]. 食品科技, 2015,40(10):265-269.
[8]	林连波, 符小文, 艾朝晖, 等. 海南山苦茶叶的化学成分研究Ⅰ[J]. 中国中药杂志, 2006,31(6):477-479.
[9]	林连波, 刘明生, 林强, 等. 海南山苦茶挥发油成分的研究[J]. 时珍国医国药, 2001,12(10):865-866.
[10]	李彦军, 王勇, 高艳娟, 等. 鹧鸪茶提取物抑菌活性研究及其成分分析[J]. 食品工业科技, 2014,35(10):202-204.
[11]	瞿兆蕙, 杜磊, 匡婷, 等. 低能量复合鹧鸪茶凉茶饮料的开发与抗氧化活性评价[J]. 食品工业科技, 2019,40(15):1-7, 13.
[12]	Chan C L, Gan R Y, Shah N P, et al. Polyphenols from selected dietary spices and medicinal herbs differentially affect common food-borne pathogenic bacteria and lactic acid bacteria[J]. Food Control, 2018,92:437-443.
[13]	Zamora-Ros R, Knaze V, Rothwell J A, et al. Dietary polyphenol in-take in Europe: the European prospective investigation into cancer and nutrition (EPIC) study[J]. European Journal of Nutrition, 2016,55(4):1359-1375. DOI URL PMID
[14]	Safriani N, Erfiza N M, Arpi N. Antioxidant activities of Cyperus rotundus L. rhizome and Areca catechu L. seed[J]. International Journal on Advanced Science, Engineering and Information Technology, 2016,6(3):285-288
[15]	Palmaduran S A, Vlassopoulos A, Lean M, et al. Nutritional intervention and impact of polyphenol on glycohaemoglobin (HbA1c) in non-diabetic and type 2 diabetic subjects: Systematic review and meta-analysis[J]. Critical Reviews in Food Science & Nutrition, 2017,57(5):975-986. DOI URL PMID
[16]	Gan R Y, Deng Z Q, Yan A X, et al. Pigmented edible bean coats as natural sources of polyphenols with antioxidant and antibacterial effects[J]. LWT-Food Science and Technology, 2016,73:168-177.
[17]	高海荣, 赵爱娟, 陈秀丽, 等. 从茶叶中提取茶多酚工艺的对比研究[J]. 中国食品添加剂, 2017(3):133-137.
[18]	段宙位, 窦志浩, 何艾, 等. 青金桔皮中多酚的提取及其抗氧化性研究[J]. 食品工业科技, 2015,36(10):244-248.
[19]	俞忠明, 汪立新, 王娜妮, 等. 响应面法优化复合酶提取黑蒜多酚的工艺研究[J]. 中药材, 2018,41(12):2883-2885.
[20]	张婷婷, 张宾乐, 王俗欢, 等. 超声辅助提取武夷岩茶茶梗中茶多酚的工艺优化[J]. 食品工业科技, 2019,40(16):140-144, 150.
[21]	Cai H, Xie Z Y, Liu G H, et al. Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun[J]. PLoS One, 2014,9(3):1-14.
[22]	Dinh T V, Saravana P S, Woo H C, et al. Ionic liquid-assisted subcritical water enhances the extraction of phenolics from brown seaweed and its antioxidant activity[J]. Separation and Purification Technology, 2018,196:287-299. DOI URL
[23]	朱淑云, 周越, 肖香, 等. 水飞蓟油的体外抗氧化活性及对氧化损伤小鼠的保护作用[J]. 食品科学, 2018,39(5):234-238.
[24]	李花, 陈国德, 李培征, 等. 海南4 种茶水提物的抗氧化及抑菌活性比较[J]. 食品工业科技, 2018,39(23):56-63.
[25]	郭俊花, 张增帅, 马欣, 等. 11种食药同源植物提取物对果蔬常见腐败菌的抑菌活性研究[J]. 天然产物研究与开发, 2019,31:2025-2031.
[26]	李钟美, 黄和. 高良姜提取物抑菌活性及稳定性研究[J]. 食品与机械, 2016,32(2):55-59
[27]	Kwon T H, Kim T W, Kim C G,et al. Antioxidant activity of various solvent fractions from edible brown alga, eisenia bicyclis and its active compounds[J]. Journal of Food Science, 2013,78(5):679-684.
[28]	胡长玉, 戴毅, 王雅群, 等. 祁白术多酚提取工艺及抗氧化、抑菌活性研究[J]. 食品与机械, 2019,35(1):170-176.
[29]	张秀芬, 何文, 谢君锋, 等. 辣木茶多酚的提取工艺优化及其抗氧化活性研究[J]. 化学与生物工程, 2019,36(4):37-41.
[30]	魏园园, 刘琪, 梁宗瑶, 等. 栓皮栎橡子果仁多酚抗氧化与抑菌活性研究[J]. 食品工业科技, 2019,40(20):42-48.
[31]	张昊, 任发政. 羟基和超氧自由基的检测研究进展[J]. 光谱学与光谱分析, 2009,29(4):1093-1099.