Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (3): 847-853.DOI: 10.3969/j.issn.1000-2561.2021.03.033

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Antioxidant and Bacteriostasis Activity of Flavanoid from Mallotus oblongifolius by Different Extraction Methods

DUAN Zhouwei1,2, LI Peng3, HE Ai1, WANG Shiping1, XIE Hui1,*()   

  1. 1. Institute of Processing & Design of Agroproducts, Hainan Academy of Agricultural Science, Haikou, Hainan; 571100, China
    2. Collage of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
    3. College of Animal Science and Technology, Hainan University, Haikou, Hainan 570228, China
  • Received:2020-03-28 Revised:2020-05-13 Online:2021-03-25 Published:2021-03-25
  • Contact: XIE Hui


The aim was to select the suitable method for extracting the polyphenols from Mallotus oblongifolius. The polyphenols were extracted by hot water extraction, organic solvent extraction and cellulase-assisted extraction. The antioxidant activity of the extract density was evaluated by scavenging DPPH, ABTS, hydroxyl, superoxide anion radical and reducing force methods. The antibacterial activity of the polyphenol extracts was determined by filter paper diffusion method and double dilution methods. The yield of polyphenols by the hot water extraction method, organic solvent extraction method and cellulase-assisted extraction method was (10.31±0.42)%, (9.79±0.38)%, and (10.88± 0.52)%, respectively, and the content was (44.72±2.26)%, (39.54±1.78)%, and (47.07±2.52)%. The polyphenol extracts extracted by the three methods had strong antioxidant properties. The cellulose-assisted extraction of the polyphenols from M. oblongifolius (CFC) had the strongest ability to scavenge DPPH radical, and its half inhibitory concentration (IC50) was (0.0034±0.0002) mg/mL. The hot water-extracted polyphenols from M. oblongifolius (WFC) had the strongest ability to scavenge ABTS radical, hydroxyl radical, and superoxide anion radical, and the IC50 values was (0.066±0.004), (0.069±0.004), (0.127±0.009) mg/mL. The polyphenol extracts extracted by the three methods had a good inhibitory effect on four bacteria. Among them, WFC had a good inhibitory effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aerugino, the inhibitory zone diameter was (12.34±1.01), (12.16±0.95), (2.12±0.15), (6.12±0.36)mm, and the minimum inhibitory concentration (MIC) was 3.13, 3.13, 12.50, 6.25 mg/mL. Comprehensive consideration, the hot water extraction method is more suitable for the extraction of polyphenols from M. oblongifolius.

Key words: Mallotus oblongifolius, flavonoids, extraction, antioxidant activity, antibacterial

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