Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (3): 822-829.DOI: 10.3969/j.issn.1000-2561.2021.03.030

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Identification and Biological Characteristics of Fusarium solani Causing Coffee Black Berry Disease

ZHU Mengfeng1, WU Weihuai2,*(), HE Chunping2, LIANG Yanqiong2, LU Ying2, XI Jingen2, MO Lizhen3, TAN Shibei2, YI Kexian2,*()   

  1. 1. School of Life Science and Pharmacy, Hainan University, Haikou, Hainan 570288, China
    2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101
    3. Yunnan Pu’er Tea and Coffee Industry Development Center, Pu’er, Yunnan 665000, China
  • Received:2020-05-05 Revised:2020-06-16 Online:2021-03-25 Published:2021-03-25
  • Contact: WU Weihuai,YI Kexian

Abstract:

In this study, the pathogen was isolated from an unknown disease in the coffee garden in Yunnan, which can darken the coffee berry. Two strains, CPE5 and CPE12 were obtained. The strains had a round, felt-like colony on PDA medium, the mycelium was off-white, the surface was sparse, and light yellow pigment appeared on the back. The conidia had 1-8 septums, its length was 6.08-65.3 μm and width was 2.76-9.03 μm. Small conidia were kidney-shaped and large conidia were sickle-shaped. The pathogenicity test showed that no matter whether inoculating healthy fresh coffee leaves or fruits, the symptoms and the morphological characteristics of the conidia obtained after re-isolation were consistent with the original inoculated strains. Molecular identification results showed that ITS, β-tubulin, TEF, 28S rDNA, four single gene clustering tree, and ITS-TEF adduct gene sequence clustering results were consistent, indicating that CPE5 and CPE12 belong to Fusarium solani. This is the first report that Fusarium solani harms coffee berry. Studies on the biological characteristics of the pathogen showed that the most suitable growth media were PDA and corn flour agar medium, the optimum growth temperature was 28 ℃, full light was conducive to the growth. The strains had the highest utilization rate of carbon source mannitol and nitrogen source beef extract, glycine, and urea. The strains could be sterilized at 75 ℃ for 10 minutes. The EC50 values of prochloraz manganese salt and tebuconazole were 1.8352 μg/mL and 1.4826 μg/mL, respectively, which had a very significant inhibitory effect on the growth of CPE5 mycelium.

Key words: coffee, Fusarium solani, molecular identification, biological characteristics

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