Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (1): 198-204.DOI: 10.3969/j.issn.1000-2561.2021.01.027

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Screening of Proteins Interacting with CsSSK1 in Colletotrichum siamense by Yeast Two-hybrid Technique

FANG Siqi, LI Zedong, WANG Jiyuan, HE Qiguang, LI Xiao, LIU Wenbo, ZHANG Yu, LIN Chunhua*, MIAO Weiguo*   

  1. College of Plant Protection, Hainan University / Key Laboratory of Green Forest Control and Prevention, Ministry of Education, Haikou, Hainan 570228, China
  • Received:2019-11-06 Revised:2020-03-11 Online:2021-01-25 Published:2021-01-25
  • Contact: LIN Chunhua,MIAO Weiguo


Colletotrichum siamense is the main pathogen of anthracnose of rubber tree or other tropical crops. A two component system is involved in the adaptation of fungi to external environmental changes, drug resistance and virulence in pathogenic fungi. In pathogenic fungi, it includes receptors and SSK1 (SLF-interacting SKP1-like1) response regulatory protein components. To obtain proteins interacting with SSK1 in C. siamense, a homologue gene CsSSK1, 2750 bp long with cDNA 2190 bp, was cloned. It contained a cheY homologs receiving domains and 10 complex structures. Then the pGBKT7-CsSSK1 vector was constructed. By an yeast two hybrid system, ten proteins, including transketolase, β-lactamase, alcohol dehydrogenase, ubiquitin, tubulin and 5 unknown or hypothetical proteins, maight interacting with CsSSK1, were identified after sequencing and bioinformatics analysis. This result would provide a basis for further study of the functions, interaction proteins and regulatory mechanism of CsSSK1.

Key words: Colletotrichum siamense, two component system, response regulatory protein SSK1, interaction protein

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