Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (12): 2507-2512.DOI: 10.3969/j.issn.1000-2561.2020.12.019

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Biological Characteristics of Momordica charantia Leaf Spot Pathogen Bipolaris bicolor and Bacteriostatic Agents Screening

QIN Jian1,2, HUANG Rukui1,2,*(), HUANG Xiongjuan1,2, LIU Xinglian1, LIANG Jiazuo1, JU Xixi1,2, HUANG Yuhui1,2   

  1. 1. Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
    2. Guangxi Key Laboratory of Vegetable Breeding and New Technology Development, Nanning, Guangxi 530007, China
  • Received:2020-01-09 Revised:2020-02-29 Online:2020-12-25 Published:2020-12-25
  • Contact: HUANG Rukui

Abstract:

To clarify the biological characteristics and sensitivity to different fungicides, the effects of different culture condition on the mycelial growth of Bipolaris bicolor were determined. The inhibitory activity of ten fungicides on B. bicolor were also studied. The optimum temperature and pH for the mycelial growth was 25-30 ℃ and pH 6-7, respectively. PSA and CMA medium were the appropriate medium for hyphal growth. Continuous darkness condition and the alternation of light and darkness were beneficial to mycelial growth. Effects for carbon and nitrogen sources on mycelial growth were significantly different, and the optimal carbon and nitrogen source was dextrose and peptone, respectively. The lethal temperature of mycelia was 45 ℃. The toxicity test showed that 250 g/L difenoconazole EC had the highest inhibitory activity with EC50 of 0.0233 mg/L among all fungicides, followed by 250 g/L pyraclostrobin SC, 20% imazalil EW and 50% cyprodinil WDG.

Key words: Momordica charantia, leaf spot, Bipolaris bicolor, biological characteristics, toxicity

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