Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (11): 2335-2345.DOI: 10.3969/j.issn.1000-2561.2020.11.027
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MU Zhihua1,2,LI Zhiying1,LIU Rui1,FAN Haikuo1,*()
Received:
2020-07-10
Revised:
2020-08-20
Online:
2020-11-25
Published:
2020-12-23
Contact:
FAN Haikuo
CLC Number:
MU Zhihua,LI Zhiying,LIU Rui,FAN Haikuo. The Development of Coconut (Cocos nucifera L.) Tissue Culture Technology in the Past 40 Years: a Review[J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2335-2345.
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文献来源 Reference | 外植体 Explant | 培养基Medium | ||||
---|---|---|---|---|---|---|
基础培养基 Basal medium | 愈伤诱导过程中激素添加 Hormone during induction | 体胚成熟过程中激素添加 Hormone during maturation | ||||
Eeuwens等[ | 未成熟花序、穗轴及未成熟茎 | Y3 | ||||
Pannetier等[ | 幼嫩叶片 | Eeuwens基础培养基+3%蔗糖+活性炭 | MW维生素+2,4-D | |||
Branton等[ | 未成熟花序、根、叶、未成熟茎 | MS大量元素+Y3微量元素+Blake维生素+50 g/L蔗糖+2.5 g/L活性炭 | 10-4mol/L 2,4-D+5 μmol/L BAP+5 μmol/L 2iP | 0.1 μmol/L 2,4-D | ||
Gupta等[ | 成熟胚、茎、穗轴、叶片 | Y3培养基+2.5 g/L活性炭 | 452 μmol/L 2,4-D+26.9 μmol/ L NAA+8.88 μmol/L BAP+4.65 μmol/L KT | 2.3 μmol/L 2,4-D+5.38 μmol/L NAA+4.44 μmol/L BAP+2.32 μmol/L KT | ||
Bhalla-Sarin等[ | 8~10个月大的成熟胚 | B5培养基 | 2 mg/L IAA | 2.0 mg/L IAA-asp+2.0 mg/ L Kn或2.0 mg/L NAA | ||
Karunaratne等[ | 6~8个月大的未 成熟胚 | De Fossard培养基+30 g/L蔗糖+ 2.5 g/L活性炭 | 12~20 μmol/L 2,4-D | 8 μmol/L 2,4-D+10 μmol/L BAP/KT | ||
Verdeil等[ | 未成熟花序 | Y3培养基+116.8 mmol/L蔗糖+2 g/L活性炭 | MW维生素+(1.5~3.5)× 10-4 mol/L 2,4-D | 2,4-D+BAP | ||
Hornung[ | 胚芽 | MS培养基 | 2,4-D | |||
Magnaval等[ | 未成熟花序 | MS大量元素+Nitsch微量元素+MW维生素 | 130 mg/L 2,4-D | |||
Adkins等[ | 成熟胚芽切片 | Y3培养基+90 mmol/L蔗糖+2.5 g/L 活性炭 | 125 μmol/L 2,4-D,+2 μmol/L AVG+3 μmol/L STS | 1 μmol/L精胺+7.5 μmol/L腐胺 | ||
Chan等[ | 成熟胚胚芽 | Y3培养基+2.5 g/L活性炭 | 100 μmol/L 2,4-D | 1 μmol/L 2,4-D+50 μmol/L BAP | ||
Samosir等[ | 成熟胚切片 | Y3培养基+30 g/L蔗糖+2.5 g/L活性炭 | MW维生素+125 μmol/L 2,4-D | 50 μmol/L 2,4-D+5 μmol/L 2iP+5 μmol/L BAP | ||
Fernando等[ | 未成熟胚 | BM72培养基+6% (W/V)蔗糖 | 24 μmol/L 2,4-D,+ 2.5~7.5 μmol/L ABA | 16 μmol/L 2,4-D+2.5~5 μmol/L ABA | ||
Fernando等[ | 成熟胚胚芽 | BM72培养基+6% (W/V)蔗糖+2.5 g/L活性炭 | 24 μmol/L 2,4-D | 5 μmol/L ABA | ||
Pérez-Nú?ez等[ | 成熟胚胚芽 | Y3培养基+30 g/L蔗糖+2.5 g/L活性炭 | 600 μmol/L 2,4-D | 6 μmol/L 2,4-D+ 300 μmol/L BAP | ||
文献来源 Reference | 外植体 Explant | 培养基Medium | ||||
基础培养基 Basal medium | 愈伤诱导过程中激素添加 Hormone during induction | 体胚成熟过程中激素添加 Hormone during maturation | ||||
Saenz等[ | 成熟胚胚芽 | Y3培养基+2.5 g/L活性炭 | 0.65 mmol/L 2,4-D | 6 μmol/L 2,4-D +300 mmol/L BAP | ||
Perera等[ | 花粉 | Y3培养基+90 g/L蔗糖+1 g/L活性炭 | 100 μmol/L 2,4-D | 0.1 μmol/L 2,4-D+5 μmol/L BAP+0.35 μmol/L GA3 | ||
Perera等[ | 未受精子房 | CRI72培养基+40 g/L蔗糖+1 g/L活性炭 | 100 μmol/L 2,4-D, 9 μmol/L TDZ | |||
Antonova[ | 未成熟花序 | Y3培养基 | 0.1 μmol/L精胺+7.5 mmol/L腐胺 | |||
Rajesh等[ | 成熟胚胚芽 | Y3培养基+30 g/L蔗糖+1 g/L活性炭 | 74.66 μmol/L 2,4-D+ 4.54 μmol/L TDZ | 10 mg/L 2,4-D+100 μmol/L精胺+1000 μmol/L腐胺 | ||
Kumar等[ | 未成熟胚 | Y3培养基+1 g/L活性炭 | 50 mg/L 2,4-D+ 2 mg/L KT | |||
Sandoval-Cancino等[ | 未成熟花序 | Y3培养基+30 g/L蔗糖+2.5 g/L活性炭 | 650 μmol/L 2,4-D | 6 μmol/L 2,4-D+300 μmol/L BAP | ||
Nguyen[ | 胚芽 | Y3培养基 | 600 μmol/L 2,4-D | 6 μmol/L 2,4-D+300 μmol/L BAP | ||
Sáenz等[ | 胚芽 | Y3培养基+50 g/L蔗糖+3 g/L凝胶+ 2.5 g/L活性炭 | 0.6 mmol/L 2,4-D | 6 μmol/L 2,4-D+0.3 mmol/L BAP |
Tab. 1 Brief summary of coconut tissue culture techniques
文献来源 Reference | 外植体 Explant | 培养基Medium | ||||
---|---|---|---|---|---|---|
基础培养基 Basal medium | 愈伤诱导过程中激素添加 Hormone during induction | 体胚成熟过程中激素添加 Hormone during maturation | ||||
Eeuwens等[ | 未成熟花序、穗轴及未成熟茎 | Y3 | ||||
Pannetier等[ | 幼嫩叶片 | Eeuwens基础培养基+3%蔗糖+活性炭 | MW维生素+2,4-D | |||
Branton等[ | 未成熟花序、根、叶、未成熟茎 | MS大量元素+Y3微量元素+Blake维生素+50 g/L蔗糖+2.5 g/L活性炭 | 10-4mol/L 2,4-D+5 μmol/L BAP+5 μmol/L 2iP | 0.1 μmol/L 2,4-D | ||
Gupta等[ | 成熟胚、茎、穗轴、叶片 | Y3培养基+2.5 g/L活性炭 | 452 μmol/L 2,4-D+26.9 μmol/ L NAA+8.88 μmol/L BAP+4.65 μmol/L KT | 2.3 μmol/L 2,4-D+5.38 μmol/L NAA+4.44 μmol/L BAP+2.32 μmol/L KT | ||
Bhalla-Sarin等[ | 8~10个月大的成熟胚 | B5培养基 | 2 mg/L IAA | 2.0 mg/L IAA-asp+2.0 mg/ L Kn或2.0 mg/L NAA | ||
Karunaratne等[ | 6~8个月大的未 成熟胚 | De Fossard培养基+30 g/L蔗糖+ 2.5 g/L活性炭 | 12~20 μmol/L 2,4-D | 8 μmol/L 2,4-D+10 μmol/L BAP/KT | ||
Verdeil等[ | 未成熟花序 | Y3培养基+116.8 mmol/L蔗糖+2 g/L活性炭 | MW维生素+(1.5~3.5)× 10-4 mol/L 2,4-D | 2,4-D+BAP | ||
Hornung[ | 胚芽 | MS培养基 | 2,4-D | |||
Magnaval等[ | 未成熟花序 | MS大量元素+Nitsch微量元素+MW维生素 | 130 mg/L 2,4-D | |||
Adkins等[ | 成熟胚芽切片 | Y3培养基+90 mmol/L蔗糖+2.5 g/L 活性炭 | 125 μmol/L 2,4-D,+2 μmol/L AVG+3 μmol/L STS | 1 μmol/L精胺+7.5 μmol/L腐胺 | ||
Chan等[ | 成熟胚胚芽 | Y3培养基+2.5 g/L活性炭 | 100 μmol/L 2,4-D | 1 μmol/L 2,4-D+50 μmol/L BAP | ||
Samosir等[ | 成熟胚切片 | Y3培养基+30 g/L蔗糖+2.5 g/L活性炭 | MW维生素+125 μmol/L 2,4-D | 50 μmol/L 2,4-D+5 μmol/L 2iP+5 μmol/L BAP | ||
Fernando等[ | 未成熟胚 | BM72培养基+6% (W/V)蔗糖 | 24 μmol/L 2,4-D,+ 2.5~7.5 μmol/L ABA | 16 μmol/L 2,4-D+2.5~5 μmol/L ABA | ||
Fernando等[ | 成熟胚胚芽 | BM72培养基+6% (W/V)蔗糖+2.5 g/L活性炭 | 24 μmol/L 2,4-D | 5 μmol/L ABA | ||
Pérez-Nú?ez等[ | 成熟胚胚芽 | Y3培养基+30 g/L蔗糖+2.5 g/L活性炭 | 600 μmol/L 2,4-D | 6 μmol/L 2,4-D+ 300 μmol/L BAP | ||
文献来源 Reference | 外植体 Explant | 培养基Medium | ||||
基础培养基 Basal medium | 愈伤诱导过程中激素添加 Hormone during induction | 体胚成熟过程中激素添加 Hormone during maturation | ||||
Saenz等[ | 成熟胚胚芽 | Y3培养基+2.5 g/L活性炭 | 0.65 mmol/L 2,4-D | 6 μmol/L 2,4-D +300 mmol/L BAP | ||
Perera等[ | 花粉 | Y3培养基+90 g/L蔗糖+1 g/L活性炭 | 100 μmol/L 2,4-D | 0.1 μmol/L 2,4-D+5 μmol/L BAP+0.35 μmol/L GA3 | ||
Perera等[ | 未受精子房 | CRI72培养基+40 g/L蔗糖+1 g/L活性炭 | 100 μmol/L 2,4-D, 9 μmol/L TDZ | |||
Antonova[ | 未成熟花序 | Y3培养基 | 0.1 μmol/L精胺+7.5 mmol/L腐胺 | |||
Rajesh等[ | 成熟胚胚芽 | Y3培养基+30 g/L蔗糖+1 g/L活性炭 | 74.66 μmol/L 2,4-D+ 4.54 μmol/L TDZ | 10 mg/L 2,4-D+100 μmol/L精胺+1000 μmol/L腐胺 | ||
Kumar等[ | 未成熟胚 | Y3培养基+1 g/L活性炭 | 50 mg/L 2,4-D+ 2 mg/L KT | |||
Sandoval-Cancino等[ | 未成熟花序 | Y3培养基+30 g/L蔗糖+2.5 g/L活性炭 | 650 μmol/L 2,4-D | 6 μmol/L 2,4-D+300 μmol/L BAP | ||
Nguyen[ | 胚芽 | Y3培养基 | 600 μmol/L 2,4-D | 6 μmol/L 2,4-D+300 μmol/L BAP | ||
Sáenz等[ | 胚芽 | Y3培养基+50 g/L蔗糖+3 g/L凝胶+ 2.5 g/L活性炭 | 0.6 mmol/L 2,4-D | 6 μmol/L 2,4-D+0.3 mmol/L BAP |
成长阶段(时间顺序) Growth stage (in time order) | 接种培养基 Culture medium | 培养时间及条件 Culture period and conditions | 备注 Comments |
---|---|---|---|
外植体 | CRI72+160 μmol/L 2,4-D,9 μmol/L TDZ,0.1% (W/V)活性炭+4%蔗糖+0.25%凝胶 | 10周(期间无继代) | pH 5.8,28 ℃ |
初代愈伤组织(耳状结构) | |||
胚性愈伤结构 | CRI72+95 μmol/L 2,4-D | 6周继代一次,共继代2次 | 无菌环境操作 |
出芽的胚性愈伤结构 | 4周 | 将愈伤组织切分成小块 | |
发育良好的体胚 | Y3+20 μmol/L 2iP | 4周 | |
发芽的体胚 | 4周 | 诱导出苗、出根 | |
试管苗 | Y3+0.45 μmol/L GA3 | 28 ℃,16 h光照光强25 μmol/(m2·s) |
Tab. 2 Experimental process of coconut ovary regeneration system
成长阶段(时间顺序) Growth stage (in time order) | 接种培养基 Culture medium | 培养时间及条件 Culture period and conditions | 备注 Comments |
---|---|---|---|
外植体 | CRI72+160 μmol/L 2,4-D,9 μmol/L TDZ,0.1% (W/V)活性炭+4%蔗糖+0.25%凝胶 | 10周(期间无继代) | pH 5.8,28 ℃ |
初代愈伤组织(耳状结构) | |||
胚性愈伤结构 | CRI72+95 μmol/L 2,4-D | 6周继代一次,共继代2次 | 无菌环境操作 |
出芽的胚性愈伤结构 | 4周 | 将愈伤组织切分成小块 | |
发育良好的体胚 | Y3+20 μmol/L 2iP | 4周 | |
发芽的体胚 | 4周 | 诱导出苗、出根 | |
试管苗 | Y3+0.45 μmol/L GA3 | 28 ℃,16 h光照光强25 μmol/(m2·s) |
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