Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (11): 2280-2284.DOI: 10.3969/j.issn.1000-2561.2020.11.019

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Distribution and Identification of Viruses from Cucumber in Hainan Island

CHE Haiyan,CAO Xueren,HE Yanheng,LUO Daquan()   

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2019-11-08 Revised:2019-12-30 Online:2020-11-25 Published:2020-12-23
  • Contact: LUO Daquan

Abstract:

From November 2018 to March 2019, a total of 302 suspected virus-infected cucumber leaf samples were collected from 8 regions (Sanya, Ledong, Dongfang, Danzhou, Chengmai, Haikou, Wanning and Wuzhishan) in Hainan Island. Five viruses were detected including Cucumber mosaic virus (CMV), Melon yellow spot virus (MYSV), Cucumber green mottle mosaic virus (CGMMV), Tobacco mosaic virus (TMV) , and Cucurbit chlorotic yellows virus (CCYV) by small RNA deep sequencing and RT-PCR. The detection rate was 32.79%, 31.46%, 23.18%, 9.61% and 2.65%, respectively. Detection rate of co-infection was 11.28%. There were 8 kinds of co-infection types, and the co-infection of two viruses occurred most often. The distribution and species of viruses from cucumber in Hainan were clarified.

Key words: Hainan Island, cucumber virus disease, small RNA deep sequencing, pathogen identification, distribution

CLC Number: