Effects of Different Cassava Cultivars on Antioxidant Enzyme Activities of Paracoccus marginatus

doi:10.3969/j.issn.1000-2561.2020.11.018

Abstract

Abstract:

To study the selective mechanism of Paracoccus marginatus to different cassava cultivars, the activity change of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and polyphenol oxidase (PPO) in P. marginatus after feeding on different cassava cultivars for 1, 2 and 4 days were determined by spectrophotometry. The results showed that the activity of antioxidant enzyme showed significant difference while P. marginatus feeding on different cassava cultivars. The activity of SOD in P. marginatus was the lowest after feeding on ‘C1115’, the activity of CAT was the lowest after feeding on ‘SC8002’, and the activity of POD and PPO was the lowest after feeding on ‘SC8’. Furthermore, the activity of SOD, CAT, POD and PPO decreased significantly compared to those of the control after feeding on the ‘J1301’ ‘SC11’ ‘SC8002’ ‘Dongguan Hongwei’ ‘SC8’ ‘Myanmar’ and ‘C1115’ at any analysis time. The above cassava cultivars could inhibit the activity of antioxidant enzymes of P. marginatus, which could interfere their normal reactive oxygen species metabolism and probably possessed the resistance potential to P. marginatus .

Key words: Paracoccus marginatus, cassava cultivars, antioxidant enzyme, activity change

CLC Number:

CHEN Qian,LIANG Xiao,WU Chunling,CHEN Qing,LIU Ying,ZHANG Zhe. Effects of Different Cassava Cultivars on Antioxidant Enzyme Activities of Paracoccus marginatus[J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2273-2279.

Figures/Tables 4

References 27

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品种 Cultivars	取食后酶活性 Activity after feeding/(U·mL^-1)			取食后与对照的酶活性比率 Ratio of enzyme activity after feeding to controls/%
品种 Cultivars	1 d (P=0.04)	2 d (P=0.07)	4 d (P=0.06)	1 d (P=0.04)	2 d (P=0.10)	4 d (P=0.16)
CK	114.01±3.32^A	122.47±3.18^A	110.33±3.87^A
面包木薯	116.34±1.74^A	114.87±1.64^B	111.06±1.93^A	102.10±2.31^A	93.82±2.00^A	100.76±4.04^A
糯米木薯	103.45±1.56^C	104.93±1.11^D	107.87±1.74^B	90.79±1.32^BC	85.72±3.21^BC	97.85±3.06^AB
ZM9066	109.53±0.92^B	104.93±0.74^D	106.77±1.74^B	96.13±1.85^AB	85.70±2.08^BC	96.86±3.51^AB
瑞士T7	105.66±1.84^B	102.53±1.66^DE	104.00±1.93^C	92.72±1.08^BC	83.74±1.73^C	94.34±3.21^AB
SC205	110.20±1.43^B	109.34±1.84^C	102.90±1.18^CD	96.72±1.55^AB	89.30±1.73^B	93.35±3.51^AB
GR5	103.27±0.92^C	95.91±0.92^F	100.88±1.74^DE	90.62±1.09^BC	78.35±2.65^D	91.52±3.51^B
SC6068	98.30±4.10^D	101.24±1.11^E	99.59±1.30^E	86.31±2.99^C	82.69±2.08^C	90.33±3.21^B
J1301	92.78±4.79^E	88.91±0.55^GH	91.49±1.56^F	81.38±2.11^D	72.62±1.53^EF	82.99±2.65^C
SC11	99.22±1.19^D	87.62±1.11^HI	89.65±1.19^F	87.08±1.61^C	71.58±2.31^EF	81.33±3.00^CD
SC8002	74.92±1.18^G	85.04±0.37^I	86.33±1.56^G	65.75±1.23^F	69.47±2.08^FG	78.31±2.52^D
东莞红尾	87.44±2.40^F	90.94±2.21^G	85.04±1.11^GH	76.72±1.19^E	74.26±1.53^E	77.16±3.06^D
SC8	76.97±2.77^G	79.89±1.37^JK	83.76±1.29^HI	90.98±2.43^F	65.26±1.53^GH	75.97±2.31^DE
缅甸种	76.39±1.19^G	77.13±0.55^K	82.28±0.92^I	67.05±2.22^F	63.01±1.73^H	74.64±2.08^DE
C1115	78.60±0.93^G	80.44±1.19^J	76.39±1.56^J	68.98±1.04^F	65.71±1.53^GH	69.31±3.06^E

品种 Cultivars	取食后酶活性 Activity after feeding/(U·mL^-1)			取食后与对照的酶活性比率 Ratio of enzyme activity after feeding to controls/%
品种 Cultivars	1 d (P=0.04)	2 d (P=0.07)	4 d (P=0.06)	1 d (P=0.04)	2 d (P=0.10)	4 d (P=0.16)
CK	114.01±3.32^A	122.47±3.18^A	110.33±3.87^A
面包木薯	116.34±1.74^A	114.87±1.64^B	111.06±1.93^A	102.10±2.31^A	93.82±2.00^A	100.76±4.04^A
糯米木薯	103.45±1.56^C	104.93±1.11^D	107.87±1.74^B	90.79±1.32^BC	85.72±3.21^BC	97.85±3.06^AB
ZM9066	109.53±0.92^B	104.93±0.74^D	106.77±1.74^B	96.13±1.85^AB	85.70±2.08^BC	96.86±3.51^AB
瑞士T7	105.66±1.84^B	102.53±1.66^DE	104.00±1.93^C	92.72±1.08^BC	83.74±1.73^C	94.34±3.21^AB
SC205	110.20±1.43^B	109.34±1.84^C	102.90±1.18^CD	96.72±1.55^AB	89.30±1.73^B	93.35±3.51^AB
GR5	103.27±0.92^C	95.91±0.92^F	100.88±1.74^DE	90.62±1.09^BC	78.35±2.65^D	91.52±3.51^B
SC6068	98.30±4.10^D	101.24±1.11^E	99.59±1.30^E	86.31±2.99^C	82.69±2.08^C	90.33±3.21^B
J1301	92.78±4.79^E	88.91±0.55^GH	91.49±1.56^F	81.38±2.11^D	72.62±1.53^EF	82.99±2.65^C
SC11	99.22±1.19^D	87.62±1.11^HI	89.65±1.19^F	87.08±1.61^C	71.58±2.31^EF	81.33±3.00^CD
SC8002	74.92±1.18^G	85.04±0.37^I	86.33±1.56^G	65.75±1.23^F	69.47±2.08^FG	78.31±2.52^D
东莞红尾	87.44±2.40^F	90.94±2.21^G	85.04±1.11^GH	76.72±1.19^E	74.26±1.53^E	77.16±3.06^D
SC8	76.97±2.77^G	79.89±1.37^JK	83.76±1.29^HI	90.98±2.43^F	65.26±1.53^GH	75.97±2.31^DE
缅甸种	76.39±1.19^G	77.13±0.55^K	82.28±0.92^I	67.05±2.22^F	63.01±1.73^H	74.64±2.08^DE
C1115	78.60±0.93^G	80.44±1.19^J	76.39±1.56^J	68.98±1.04^F	65.71±1.53^GH	69.31±3.06^E

品种 Cultivars	取食后酶活性 Activity after feeding/(L?mol^-1?cm^-1)			取食后与对照的酶活性比率 Ratio of enzyme activity after feeding to controls/%
品种 Cultivars	1 d (P=0.12)	2 d (P=0.22)	4 d (P=0.08)	1 d (P=0.25)	2 d (P=0.31)	4 d (P=0.12)
对照	49.96±0.89^A	48.69±0.73^B	48.18±0.25^A
面包木薯	45.66±0.20^BC	43.90±0.41^E	48.40±0.35^A	91.41±2.08^BC	90.18±2.00^C	100.45±3.06^A
糯米木薯	47.61±1.71^B	45.70±0.59^D	44.85±0.40^B	95.28±1.53^A	93.88±2.00^B	93.07±2.65^B
ZM9066	45.06±0.40^BC	50.87±0.30^A	43.68±0.72^B	90.21±1.00^BC	104.50±1.53^A	90.66±1.15^B
瑞士T7	40.27±1.55^F	41.22±0.53^F	48.60±1.38^A	80.58±1.53^F	84.68±1.53^D	100.86±2.52^A
SC205	44.06±0.33^CD	41.03±0.53^F	45.11±0.46^B	88.20±1.73^CD	84.28±2.65^D	93.07±1.00^B
GR5	43.73±0.40^CD	47.35±0.13^C	44.52±0.40^B	87.55±2.52^CD	97.26±1.53^B	92.39±2.08^B
SC6068	46.95±1.25^BC	46.79±1.15^C	44.55±1.41^B	93.97±1.00^AB	96.11±2.65^B	92.45±2.52^B
J1301	41.93±0.75^EF	41.16±0.24^F	41.32±0.10^C	83.94±2.00^E	84.54±2.65^D	85.76±3.21^C
SC11	36.09±0.65^G	37.57±0.17^G	40.10±0.65^C	72.25±1.53^H	77.18±1.15^E	83.23±3.06^C
SC8002	33.52±0.20^H	31.08±0.79^I	31.81±0.14^F	67.11±1.53^I	63.85±1.73^F	66.01±2.52^F
东莞红尾	42.21±1.71^DF	40.96±0.44^F	40.17±0.33^C	84.47±1.53^E	84.14±1.53^D	83.37±1.53^C
SC8	34.21±0.96^H	40.10±0.66^F	35.30±0.40^D	68.47±1.00^I	82.38±1.53^D	73.25±2.65^D
缅甸种	34.44±0.26^H	38.00±1.19^G	34.77±0.33^DE	68.94±1.00^HI	78.05±2.65^E	72.16±2.52^DE
C1115	33.39±1.39^H	36.15±0.27^H	33.52±0.79^F	66.81±1.53^I	74.27±1.15^E	69.56±1.53^E

品种 Cultivars	取食后酶活性 Activity after feeding/(L?mol^-1?cm^-1)			取食后与对照的酶活性比率 Ratio of enzyme activity after feeding to controls/%
品种 Cultivars	1 d (P=0.12)	2 d (P=0.22)	4 d (P=0.08)	1 d (P=0.25)	2 d (P=0.31)	4 d (P=0.12)
对照	49.96±0.89^A	48.69±0.73^B	48.18±0.25^A
面包木薯	45.66±0.20^BC	43.90±0.41^E	48.40±0.35^A	91.41±2.08^BC	90.18±2.00^C	100.45±3.06^A
糯米木薯	47.61±1.71^B	45.70±0.59^D	44.85±0.40^B	95.28±1.53^A	93.88±2.00^B	93.07±2.65^B
ZM9066	45.06±0.40^BC	50.87±0.30^A	43.68±0.72^B	90.21±1.00^BC	104.50±1.53^A	90.66±1.15^B
瑞士T7	40.27±1.55^F	41.22±0.53^F	48.60±1.38^A	80.58±1.53^F	84.68±1.53^D	100.86±2.52^A
SC205	44.06±0.33^CD	41.03±0.53^F	45.11±0.46^B	88.20±1.73^CD	84.28±2.65^D	93.07±1.00^B
GR5	43.73±0.40^CD	47.35±0.13^C	44.52±0.40^B	87.55±2.52^CD	97.26±1.53^B	92.39±2.08^B
SC6068	46.95±1.25^BC	46.79±1.15^C	44.55±1.41^B	93.97±1.00^AB	96.11±2.65^B	92.45±2.52^B
J1301	41.93±0.75^EF	41.16±0.24^F	41.32±0.10^C	83.94±2.00^E	84.54±2.65^D	85.76±3.21^C
SC11	36.09±0.65^G	37.57±0.17^G	40.10±0.65^C	72.25±1.53^H	77.18±1.15^E	83.23±3.06^C
SC8002	33.52±0.20^H	31.08±0.79^I	31.81±0.14^F	67.11±1.53^I	63.85±1.73^F	66.01±2.52^F
东莞红尾	42.21±1.71^DF	40.96±0.44^F	40.17±0.33^C	84.47±1.53^E	84.14±1.53^D	83.37±1.53^C
SC8	34.21±0.96^H	40.10±0.66^F	35.30±0.40^D	68.47±1.00^I	82.38±1.53^D	73.25±2.65^D
缅甸种	34.44±0.26^H	38.00±1.19^G	34.77±0.33^DE	68.94±1.00^HI	78.05±2.65^E	72.16±2.52^DE
C1115	33.39±1.39^H	36.15±0.27^H	33.52±0.79^F	66.81±1.53^I	74.27±1.15^E	69.56±1.53^E

品种 Cultivars	取食后酶活性 Activity after feeding/(U?g^-1?min^-1)			取食后与对照的酶活性比率 Ratio of enzyme activity after feeding to controls/%
品种 Cultivars	1 d (P=0.16)	2 d (P=0.13)	4 d (P=0.44)	1 d (P=0.18)	2 d (P=0.056)	4 d (P=0.056)
对照	165.87±5.90^B	159.73±6.22^BC	154.40±5.77^A
面包木薯	150.93±1.67^C	161.33±2.57^BC	146.13±1.67^B	91.10±4.36^BC	101.07±2.52^BC	94.76±4.73^A
糯米木薯	152.40±2.10^C	153.20±1.40^C	150.40±4.80^AB	91.94±2.52^B	96.00±3.61^C	97.45±2.65^A
ZM9066	138.80±2.01^D	136.27±3.03^D	130.40±1.60^C	83.78±4.04^C	85.43±4.51^D	84.52±2.52^B
瑞士T7	149.20±3.60^C	133.87±3.33^D	155.20±2.40^A	89.99±1.73^BC	83.86±2.65^D	100.64±4.93^A
SC205	139.49±4.11^D	136.53±2.40^D	127.20±1.60^C	84.16±5.51^BC	85.42±2.52^D	82.48±3.79^BC
GR5	168.40±4.40^AB	178.00±3.60^A	149.20±2.80^AB	101.57±2.31^A	111.49±2.31^A	96.76±5.20^A
SC6068	174.80±2.00^A	168.40±1.20^B	148.80±3.20^AB	105.50±4.62^A	105.51±3.21^AB	96.50±5.77^A
J1301	105.20±1.40^E	109.20±2.80^F	75.20±4.00^H	63.47±2.08^DE	68.39±1.15^E	48.80±4.36^EF
SC11	108.40±3.60^E	118.80±2.00^E	122.40±1.60^D	65.37±1.53^DE	74.42±1.53^E	79.37±4.04^CD
SC8002	104.40±2.02^E	95.20±3.20^G	68.00±3.20^HI	62.97±1.53^DE	59.62±2.52^F	44.05±1.53^F
东莞红尾	96.00±3.20^F	115.60±1.20^EF	112.00±1.80^F	57.96±3.79^EF	72.46±3.51^E	72.60±2.52^D
SC8	62.80±2.80^G	64.80±3.60^I	65.20±5.20^I	37.92±2.65^G	39.35±2.52^F	42.33±5.13^F
缅甸种	112.40±4.40^E	114.40±2.40^EF	115.20±2.40^E	67.87±4.73^D	71.65±1.15^E	74.65±2.08^D
C1115	88.40±1.20^F	80.40±4.40^H	86.40±2.40^G	53.35±2.31^F	50.46±4.51^G	56.04±3.46^E