Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (11): 2232-2236.DOI: 10.3969/j.issn.1000-2561.2020.11.012

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Effects of pH on Laccase Activity of Lentinula edodes, Pholiota nameko, and Flammulina velutipes Grown in Different State Media

ZHANG Weirui1,2(),LIU Shengrong1,2,ZHOU Xiuzhao3,RUAN Junfeng1,2,CHEN Weiwei1,CUI Xiaowei1   

  1. 1. School of Life Science, Ningde Normal University, Ningde, Fujian 352100, China
    2. Fujian Higher Education Research Center for Local Biological Resources, Ningde, Fujian 352100, China
    3. Edible Fungus Management Stations, Pingnan, Ningde, Fujian 352100, China
  • Received:2019-10-15 Revised:2020-01-21 Online:2020-11-25 Published:2020-12-23


Laccase is a key enzyme involved in the degradation and utilization of lignin and has a relationship with mushroom cultivation cycle and product quality. In this work, NaOH was used as the alkalization agent for both solidified media and liquid media, and shell ash was used for solid media, the effect of pH on the activity of laccase of Lentinula edodes, Pholiota nameko, and Flammulina velutipes grown in different state media was investigated for providing a basis for their cultivation. Results showed that the micro-alkalization of all tested culture media could improve the activity of laccase of the species, and its effect was related to media type. The optimal pH values of solidified, liquid, and solid media for L. edodes laccase activity was 6.5, 6.5 and 6.0, respectively, with a 5.3%-14.0% increase of laccase activity in relative to their controls, and for that for F. velutipes was 7.0, 6.0, and 6.5, and the increase in the activity under optimal pH values was 35.9%-343.0% compared with their controls. As to P. nameko, pH 6.0 was optimal for all three media for maximizing laccase activity, 3.1%-94.9% higher than those of their controls.

Key words: pH value, laccase, Lentinula edodes, Pholiota nameko, Flammulina velutipes

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