Effects of pH on Laccase Activity of Lentinula edodes, Pholiota nameko, and Flammulina velutipes Grown in Different State Media

doi:10.3969/j.issn.1000-2561.2020.11.012

Abstract

Abstract:

Laccase is a key enzyme involved in the degradation and utilization of lignin and has a relationship with mushroom cultivation cycle and product quality. In this work, NaOH was used as the alkalization agent for both solidified media and liquid media, and shell ash was used for solid media, the effect of pH on the activity of laccase of Lentinula edodes, Pholiota nameko, and Flammulina velutipes grown in different state media was investigated for providing a basis for their cultivation. Results showed that the micro-alkalization of all tested culture media could improve the activity of laccase of the species, and its effect was related to media type. The optimal pH values of solidified, liquid, and solid media for L. edodes laccase activity was 6.5, 6.5 and 6.0, respectively, with a 5.3%-14.0% increase of laccase activity in relative to their controls, and for that for F. velutipes was 7.0, 6.0, and 6.5, and the increase in the activity under optimal pH values was 35.9%-343.0% compared with their controls. As to P. nameko, pH 6.0 was optimal for all three media for maximizing laccase activity, 3.1%-94.9% higher than those of their controls.

Key words: pH value, laccase, Lentinula edodes, Pholiota nameko, Flammulina velutipes

CLC Number:

S646

ZHANG Weirui,LIU Shengrong,ZHOU Xiuzhao,RUAN Junfeng,CHEN Weiwei,CUI Xiaowei. Effects of pH on Laccase Activity of Lentinula edodes, Pholiota nameko, and Flammulina velutipes Grown in Different State Media[J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2232-2236.

Figures/Tables 3

References 24

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pH	漆酶活性Laccase activity/(U?L^-1)
pH	固化培养基 Solidified medium	液体培养基 Liquid medium	固体培养基 Solid medium
5.5	91.72±0.6520^d	70.72±2.3604^c	102.16±3.3054^b
6.0	95.82±0.3748^b	73.97±1.0424^b	107.59±1.0145^a
6.5	104.52±0.4111^a	78.78±0.8253^a	100.90±2.0478^bc
7.0	94.66±0.1162^c	73.51±1.3180^b	98.67±0.8785^c

pH	漆酶活性Laccase activity/(U?L^-1)
pH	固化培养基 Solidified medium	液体培养基 Liquid medium	固体培养基 Solid medium
5.5	91.72±0.6520^d	70.72±2.3604^c	102.16±3.3054^b
6.0	95.82±0.3748^b	73.97±1.0424^b	107.59±1.0145^a
6.5	104.52±0.4111^a	78.78±0.8253^a	100.90±2.0478^bc
7.0	94.66±0.1162^c	73.51±1.3180^b	98.67±0.8785^c

pH	漆酶活性Laccase activity/(U?L^-1)
pH	固化培养基 Solidified medium	液体培养基 Liquid medium	固体培养基 Solid medium
5.5	78.66±1.3759^c	38.44±5.8885^b	98.56±0.7492^b
6.0	93.28±5.5426^a	74.90±3.2757^a	101.61±1.8278^a
6.5	86.50±1.6637^b	21.33±4.0283^c	96.04±0.5373^c
7.0	74.11±1.2710^c	14.04±1.6692^d	92.58±1.2289^d

pH	漆酶活性Laccase activity/(U?L^-1)
pH	固化培养基 Solidified medium	液体培养基 Liquid medium	固体培养基 Solid medium
5.5	78.66±1.3759^c	38.44±5.8885^b	98.56±0.7492^b
6.0	93.28±5.5426^a	74.90±3.2757^a	101.61±1.8278^a
6.5	86.50±1.6637^b	21.33±4.0283^c	96.04±0.5373^c
7.0	74.11±1.2710^c	14.04±1.6692^d	92.58±1.2289^d

pH	漆酶活性Laccase activity/(U?L^-1)
pH	固化培养基 Solidified medium	液体培养基 Liquid medium	固体培养基 Solid medium
5.5	45.67±2.3997^d	5.83±0.8105^c	5.1900±0.3611^c
6.0	56.63±5.8061^c	25.76±1.1389^a	6.2850±0.3371^b
6.5	66.08±5.5889^b	9.98±1.3742^b	7.0475±0.1431^a
7.0	79.94±2.2900^a	6.32±0.5476^c	6.4175±0.3392^b