Abstract:

Substrate plays an important role in the artificial domestication and cultivation of Dendrobium sinense seedlings by affecting the water and fertilizer absorption and root aeration, and then affecting the growth of D. sinense seedlings. In this experiment, the tissue culture plantlet of D. sinense was used as the research object, and four media were used to test the plantlet. By measuring the survival rate, morphological index, strong seedling index and photosynthetic index of the tissue culture plantlet, the suitable media was selected. The results showed that the porosity and air-water ratio of 16.3 g water moss packed in 5 cm transparent plastic cup were higher, which was 42.1% and 2.51; the water conservation and aeration were good, which were beneficial to the rooting and sprouting of the tissue cultured seedlings of D. sinense. The survival rate of the tissue cultured seedlings was higher, which was 56.3%, and the strong seedling index was 0.016; the relative chlorophyll content and net photosynthetic rate were the highest; the porosity and air-water ratio of 6.0 g water moss per hole in the 105 hole disc were relatively low, and the survival rate of plantlets was low because of less matrix. Therefore, 16.3 g water moss in a 5 cm transparent plastic cup can be used as a better substrate for the cultivation of D. sinense.

Key words: Dendrobium sinense, tissue culture seedling, acclimation, cultivated matrix, photosynthetic physiology