Genetic Diversity Analysis of the Potted Chrysanthemum Based on Phenotype and SRAP Marker
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (11): 2156-2164.DOI: 10.3969/j.issn.1000-2561.2020.11.003

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Genetic Diversity Analysis of the Potted Chrysanthemum Based on Phenotype and SRAP Marker

SHEN Yao,WANG Hanxuan,HOU Haixian,WU Zhiming,ZHOU Hougao()   

  1. School of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China
  • Received:2019-12-24 Revised:2020-01-27 Online:2020-11-25 Published:2020-12-23
  • Contact: ZHOU Hougao


In order to better protect chrysanthemum germplasm resources and lay a foundation for hybrid breeding, the genetic diversity of 30 potted chrysanthemum varieties were studied based on 20 morphological and SRAP markers. The results showed that the phenotypic variation of potted chrysanthemum varieties was abundant. The coefficient of variation of characters was ranged from 14.67% to 78.25%, which were crown width and tongue floret number. The results of principal component analysis showed that the contribution rate of the first five principal components was 76.68%, which comprehensively reflected the importance of flower size, leaf shape and flower color. Based on the cluster analysis of phenotypic traits, 30 potted chrysanthemum cultivars could be divided into three groups. GroupⅠ, GroupⅡ and Group Ⅲ consisted of 14, 2 and 14 varieties correspondingly. The clustering results based on phenotypic traits were related to flower diameter and natural florescence period. Based on SRAP marker technology, 11 pairs of polymorphic primers were screened out and a total of 1866 polymorphic loci were obtained, with the polymorphic proportion as high as 92.61%. The genetic similarity coefficient between cultivars was ranged from 0.67 to 0.85, indicating that there were some genetic variations in the 30 cultivars. The samples could be divided into three groups based on the NJ tree. GroupⅠ, Group Ⅱ and Group Ⅲ consisted 17, 10 and 3 varieties correspondingly. However, the clustering results of the two grouping techniques showed that there was no good correlation between the two markers, which might be related to the complex genetic background of chrysanthemum materials and the limitations of experimental selection.

Key words: potted chrysanthemum, genetic diversity, phenotype, SRAP marker, cluster analysis

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