Association Studies of Plant Architecture Traits and SSR Markers in Nelumbo Adans.

doi:10.3969/j.issn.1000-2561.2020.11.002

Abstract

Abstract:

Totally 129 lotus accessions with different architecture were used as the experimental material. The leaf height, flower height and leaf diameter were determined. The accessed lotus population had diverse plant architecture traits. Variation coefficient for leaf height and flower height was 39.68% and 41.69% respectively, and variation coefficient for leaf length and width was 29.70% and 31.41% respectively. 129 lotus accessions were genotyped with 98 pair polymorphic SSR primers. Population structure of lotus accessions was analyzed using the STRUCTURE V2.2 software. Association analysis of lotus architecture traits was performed with the GLM model. The results indicated that lotus accessions were divided into four subgroups. The results distinctly grouped lotus accessions from Chinese lotus and Sino-American hybrids into different clusters. However, the hybrids dispersed among clusters. The results showed that 11 SSR markers were associated with leaf height, flower height, leaf length and width; three markers were associated with all the plant architecture traits, and that might be caused by multiple effects of one gene or genetic linkage. In two years of repeated tests, two markers (SSR132 and SSR458) can be associated with leaf length and width, indicating that these two markers may be less affected by environment.

Key words: Nelumbo, plant architecture, population structure, association analysis

CLC Number:

S813.3

YANG Dan,DU Fengfeng,CHANG Yajun,CUI Jian,SUN Linhe,LIU Xiaojing,YAO Dongrui. Association Studies of Plant Architecture Traits and SSR Markers in Nelumbo Adans.[J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2149-2155.

Figures/Tables 7

References 22

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编号 Number	品种名 Cultivar name	编号 Number	品种名 Cultivar name	编号 Number	品种名 Cultivar name	编号 Number	品种名 Cultivar name
1	黑龙江野生红莲	34	琴台歌手13	67	小莲座13	100	红蜻蜓
2	绍兴红莲	35	丹娘13	68	满江红13E	101	光辉
3	庐山红莲	36	金陵惜月	69	童乐	102	红日
4	北京白花藕	37	牡丹仙子	70	琴韵	103	卓越13
5	兴城藕	38	巧珠	71	花蕊夫人	104	富贵莲14B
6	小碧台	39	红碗莲	72	粉叠珠	105	斑桃
7	粉川台	40	白玉	73	露华浓13	106	复瓣瑰丽
8	单瓣红万万	41	伯里王子	74	霓裳小曲13C	107	金陵火都
9	红樱莲	42	火花	75	秦淮翡翠	108	朱笔
10	佛座莲	43	秦淮彩灯	76	洋贵人	109	巨无霸13
11	扬州碗莲	44	霓裳小曲13B	77	大师	110	台城翡翠
12	宝应藕莲	45	粉玲珑13	78	江南烟雨	111	红台莲
13	青莲姑娘	46	雪翠	79	珠峰翠影	112	绯桃
14	逸仙莲	47	秦淮白玉	80	六朝玉叶	113	红唇
15	玄武红莲	48	观音莲	81	普者黑白荷	114	红鸾
16	重瓣一丈青	49	首领	82	金陵公主	115	大洒锦
17	披针粉	50	白凤冠	83	金陵女神	116	红飘带
18	兰花指粉	51	红山茶	84	红太阳	117	嵊县粉莲
19	中国古代莲	52	娇容醉杯14	85	红彩裙	118	白衣战士
20	朝霞	53	桌上莲13	86	中国红嘉兴	119	姬妃莲
21	小红菊	54	飞天	87	中国红延安	120	大毛节
22	魏小姐	55	粉婉	88	中国红韶山	121	平头紫
23	碧台莲	56	粉牡丹	89	中国红北京	122	银球
24	满江红	57	彩云	90	中国红西北坡	123	东湖红莲
25	西湖红莲	58	春暮樱桃	91	秦淮花灯	124	粉千叶
26	宝石花	59	江南碧涵	92	单瓣锦霞	125	解放红莲
27	东方红	60	小莲仙13	93	风暴13	126	点额妆
28	伯里公主	61	粉贵人	94	锦边莲	127	东湖白莲
29	粉面桃花13	62	魏夫人	95	星甸红莲	128	石家庄白莲
30	白佛手	63	红盏托珠13	96	金陵畅想	129	宜良千瓣
31	小精彩	64	红绸	97	瑶池之辰
32	杏花粉13B	65	童欢	98	瑶池之月
33	俊愉莲13B	66	童羞面	99	瑶池之云

编号 Number	品种名 Cultivar name	编号 Number	品种名 Cultivar name	编号 Number	品种名 Cultivar name	编号 Number	品种名 Cultivar name
1	黑龙江野生红莲	34	琴台歌手13	67	小莲座13	100	红蜻蜓
2	绍兴红莲	35	丹娘13	68	满江红13E	101	光辉
3	庐山红莲	36	金陵惜月	69	童乐	102	红日
4	北京白花藕	37	牡丹仙子	70	琴韵	103	卓越13
5	兴城藕	38	巧珠	71	花蕊夫人	104	富贵莲14B
6	小碧台	39	红碗莲	72	粉叠珠	105	斑桃
7	粉川台	40	白玉	73	露华浓13	106	复瓣瑰丽
8	单瓣红万万	41	伯里王子	74	霓裳小曲13C	107	金陵火都
9	红樱莲	42	火花	75	秦淮翡翠	108	朱笔
10	佛座莲	43	秦淮彩灯	76	洋贵人	109	巨无霸13
11	扬州碗莲	44	霓裳小曲13B	77	大师	110	台城翡翠
12	宝应藕莲	45	粉玲珑13	78	江南烟雨	111	红台莲
13	青莲姑娘	46	雪翠	79	珠峰翠影	112	绯桃
14	逸仙莲	47	秦淮白玉	80	六朝玉叶	113	红唇
15	玄武红莲	48	观音莲	81	普者黑白荷	114	红鸾
16	重瓣一丈青	49	首领	82	金陵公主	115	大洒锦
17	披针粉	50	白凤冠	83	金陵女神	116	红飘带
18	兰花指粉	51	红山茶	84	红太阳	117	嵊县粉莲
19	中国古代莲	52	娇容醉杯14	85	红彩裙	118	白衣战士
20	朝霞	53	桌上莲13	86	中国红嘉兴	119	姬妃莲
21	小红菊	54	飞天	87	中国红延安	120	大毛节
22	魏小姐	55	粉婉	88	中国红韶山	121	平头紫
23	碧台莲	56	粉牡丹	89	中国红北京	122	银球
24	满江红	57	彩云	90	中国红西北坡	123	东湖红莲
25	西湖红莲	58	春暮樱桃	91	秦淮花灯	124	粉千叶
26	宝石花	59	江南碧涵	92	单瓣锦霞	125	解放红莲
27	东方红	60	小莲仙13	93	风暴13	126	点额妆
28	伯里公主	61	粉贵人	94	锦边莲	127	东湖白莲
29	粉面桃花13	62	魏夫人	95	星甸红莲	128	石家庄白莲
30	白佛手	63	红盏托珠13	96	金陵畅想	129	宜良千瓣
31	小精彩	64	红绸	97	瑶池之辰
32	杏花粉13B	65	童欢	98	瑶池之月
33	俊愉莲13B	66	童羞面	99	瑶池之云

指标 Index	位点数 N_a	有效位点数N_e	Shannon 信息指数I	观测杂合度H_o	Nei’s期望杂合度H_e
最大值	16.00	7.77	2.32	1.00	0.87
最小值	2.00	1.16	0.32	0.00	0.14
平均值	5.48	2.58	1.04	0.50	0.54
总数	537.00	256.26

指标 Index	位点数 N_a	有效位点数N_e	Shannon 信息指数I	观测杂合度H_o	Nei’s期望杂合度H_e
最大值	16.00	7.77	2.32	1.00	0.87
最小值	2.00	1.16	0.32	0.00	0.14
平均值	5.48	2.58	1.04	0.50	0.54
总数	537.00	256.26

SSR标记 SSR marker	花柄高度 Flower height	立叶高度 Leaf height	立叶长径 Leaf length	立叶短径 Leaf width
SSR064			0.5364	0.5588
SSR065		0.7264
SSR067	0.4971	0.4943	0.5749	0.4875
SSR078				0.7034
SSR132	0.5818	0.5543	0.6706	0.6424
SSR203		0.4415
SSR312	0.4225
SSR339	0.4711
SSR370		0.4236	0.4379	0.3901
SSR449		0.3836	0.4156	0.361
SSR458	0.5065	0.4728	0.5644	0.5576