Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (9): 1928-1938.DOI: 10.3969/j.issn.1000-2561.2020.09.029

• Agricultural Ecology & Environmental Protection • Previous Articles     Next Articles

Cassava Rhizosphere Soil Collected by “Root Bag” Method and Its Bacteria Diversity

WEI Yundong1,2,LUO Yanchun1,2,ZHENG Hua1,2,*(),LI Jun1,2,PAN Huan1,2,LEI Kaiwen1,2,XU Chuan1,2   

  1. 1. Guangxi Institute of Subtropical Crops, Nanning, Guangxi 530001, China
    2. Guangxi Institute of Cassava, Nanning, Guangxi 530001, China
  • Received:2019-10-30 Revised:2019-12-24 Online:2020-09-25 Published:2020-10-16
  • Contact: ZHENG Hua


In order to collect cassava rhizosphere soil and study its bacteria community, and further deepen the knowledge of the micro-ecology of the rhizosphere soil, a field trial with the “root bag” method was carried out with two soil textures (clay and sandy roam) and a series of soil quantity from 100g to 500g in the root bag. Cassava plant height was significantly influenced by the soil quantity in the root bag, while cassava shoot weight was not. Soil available nutrients were strongly affected by roots when soil quantity in the root bag was only 100g, ammonium content (NH4-N) and available potassium (AK) was enriched significantly compared to bulk soil. Results from high throughout sequencing by Illumina Hiseq showed that the dominant bacterial community was Proteobacteria (31.272%), Actinobacteria (25.753%), Acidobacteria (12.761%), Chloroflexi (8.799%) et al. Results from α diversity indexes showed that the diversity of bacteria was increased in the rhizospheric soil, and the species richness was also increased in the clay. Compared to bulk soil, the soil samples collected from the inside “root bag” was relatively rich in Sphingobacteriales, Rhizobiales, Xanthomonadales, Verrucomicrobia and related phylum, class, genus and species for the clay, and for the sandy roam, rich in Sphingobacteriales and genus and species in gemmatimonadaceae. Results from Redundancy analysis (RDA) triplots demonstrated that soil available phosphorus (AP) was positively correlated to Actinobacteria and Chloroflexi, and negatively correlated to Proteobacteria; Soil available nitrogen (AN), AK, NH4-N, nitrates nitrogen (NO3-N) were negatively correlated with Gemmatimonadetes, Acidobacteria, Parcubacteria, Elusimicrobia, Armatimonadetes, TM6_Dependentiae, Chlorobi et al., and positively correlated with Firmicutes, Cyanobacteria, Fusobacteria, Bacteroidetes. In concusion, the 100g soil in the root bag could be seen as cassava rhizospheric soil according to soil nutrients and bacteria diversity. And NH4-N and AK could be regarded as the indicators as cassava rhizospheric soil. Soil microbial diversity was improved in the rhizosphere, and Sphingobacteriales was the enriched bacteria in both soil textures. Soil nutrients were correlated with some classification of soil bacterial.

Key words: cassava rhizosphere, ammonium nitrogen, available phosphorus, microbial community, Sphingobacteriales

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