Screening and Identification of Antagonistic Actinomycete Against Anthracnose Disease of Guanxi Honey Pomelo Cultivar
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (9): 1876-1882.DOI: 10.3969/j.issn.1000-2561.2020.09.022

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Screening and Identification of Antagonistic Actinomycete Against Anthracnose Disease of Guanxi Honey Pomelo Cultivar

LAI Baochun1,2,WU Zhenqiang1,DAI Ruiqing1,LIN Minghui3,LI Feng1,LIN Defeng1,WANG Jiarui1   

  1. 1. Zhangzhou Institute of Agricultural Sciences, Zhangzhou, Fujian 363005, China
    2. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou, Fujian 350003, China
    3. Anhou Forestry Station, Pinghe Forestry Bureau, Pinghe, Fujian 363700, China
  • Received:2019-10-12 Revised:2019-12-17 Online:2020-09-25 Published:2020-10-16

Abstract:

This study was conducted to screen resources for the biological control of Colletotrichum gloeosporioides which cause anthracnose disease for Guanxi honey pomelo cultivar. An antagonistic actinomycete of C. gloeosporioides was isolated from the soil samples by the plate-confrontation method and growth speed rate method, and the inoculated fruit was treated with the fermented broth. The taxonomic identification of the strain FX28 was carried out using morphological, physiological and biochemistry characteristics and 16S rDNA sequence. From the 25 soil samples, 16 of 105 antagonistic actinomycete isolates showed againstantifungal activity against C. gloeosporioides. FX28 showed an inhibition zone with a diameter 15.3 mm and strongest inhibition rate 86.4%. FX28 had broad-spectrum inhibition activity to all the 14 plant pathogenic fungi such as C. gloeosporioides, Diaporthe citri, Phyllosticta citriasian. FX28 could break down hyphae, turn hyphal thickened, fractured and enlarged. The protection and curation effects of the fermented broth against C. gloeosporioides were up to 83.8% and 71.1%, respectively. Based on the morphological, physiological-biochemical characteristics and molecular analysis, FX28 was identified as Streptomyces violaceorubidus.

Key words: honey pomelo anthracnose disease, Streptomyces violaceorubidus, taxonomic identification, biocontrol effect

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